Photoimprint photoacoustic microscopy for three-dimensional label-free subdiffraction imaging

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Junjie Yao
  • Lidai Wang
  • Chiye Li
  • Chi Zhang
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Article number14302
Journal / PublicationPhysical Review Letters
Volume112
Issue number1
Publication statusPublished - 10 Jan 2014
Externally publishedYes

Abstract

Subdiffraction optical microscopy allows the imaging of cellular and subcellular structures with a resolution finer than the diffraction limit. Here, combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for subdiffraction photoacoustic imaging of both fluorescent and nonfluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. The differential signal between the two excitations preserves the signal contribution mostly from the center of the excitation volume, and dramatically sharpens the lateral resolution. Moreover, due to the nonlinear nature of the signal, our method offers an inherent optical sectioning capability, which is lacking in conventional photoacoustic microscopy. By scanning the excitation beam, we performed three-dimensional subdiffraction imaging of varied fluorescent and nonfluorescent species. As any molecules have absorption, this technique has the potential to enable label-free subdiffraction imaging, and can be transferred to other optical imaging modalities or combined with other subdiffraction methods. © 2014 American Physical Society.

Citation Format(s)

Photoimprint photoacoustic microscopy for three-dimensional label-free subdiffraction imaging. / Yao, Junjie; Wang, Lidai; Li, Chiye et al.
In: Physical Review Letters, Vol. 112, No. 1, 14302, 10.01.2014.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review