Photoacoustic imaging of single circulating melanoma cells in vivo

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

5 Scopus Citations
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Author(s)

  • Junjie Yao
  • Ruiying Zhang
  • Song Xu
  • Guo Li
  • Jun Zou
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9323
ISBN (print)9781628414134
Publication statusPublished - 2015
Externally publishedYes

Publication series

Name
Volume9323
ISSN (Print)1605-7422

Conference

TitlePhotons Plus Ultrasound: Imaging and Sensing 2015
PlaceUnited States
CitySan Francisco
Period8 - 10 February 2015

Abstract

Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.

Research Area(s)

  • Circulating tumor cell, Melanoma, Photoacoustic imaging

Citation Format(s)

Photoacoustic imaging of single circulating melanoma cells in vivo. / Wang, Lidai; Yao, Junjie; Zhang, Ruiying et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9323 SPIE, 2015. 93230A.

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review