TY - JOUR
T1 - Photo-responsive molecularly imprinted hydrogels for the detection of melamine in aqueous media
AU - Tang, Qian
AU - Nie, Ying-Tian
AU - Gong, Cheng-Bin
AU - Chow, Cheuk-Fai
AU - Peng, Jing-Dong
AU - Hon-Wah Lam, Michael
PY - 2012/10/7
Y1 - 2012/10/7
N2 - In this paper, by combination of the specificity of a molecularly imprinted technique, the water solubility of hydrogels and the photoisomerization property of azobenzene chromophores, we report a new and quick detection method for trace melamine in aqueous media based on photo-responsive molecularly imprinted hydrogels (MIHs). The MIHs were fabricated using a water-soluble azobenzene-containing 4-[(4-methacryloyloxy)phenylazo]benzenesulfonic acid (MAPASA) as the functional monomer, 1,3,5-benzenetriol as a mimic template, and tetramethacryloyl triethylene tetramine (TTT) as the cross-linker. The MIHs show specific affinity to melamine with a binding constant of 3.20 × 10 4 M -1 in aqueous Tris-HCl buffer at pH 7.0. The density of the melamine-specific receptor sites in the MIHs material is 2.69 μmol g -1 MIHs. Upon alternate irradiation at 365 and 440 nm, the MIHs can quantitatively uptake and release melamine. The photoisomerization rate of MIHs is related to the concentration of melamine in the detecting solution, and therefore, a quick detection method for trace melamine is established. Analytical application of the MIHs to detect the melamine concentration in both milk and milk powder has been accomplished successfully with a simple pre-treatment of the samples. © 2012 The Royal Society of Chemistry.
AB - In this paper, by combination of the specificity of a molecularly imprinted technique, the water solubility of hydrogels and the photoisomerization property of azobenzene chromophores, we report a new and quick detection method for trace melamine in aqueous media based on photo-responsive molecularly imprinted hydrogels (MIHs). The MIHs were fabricated using a water-soluble azobenzene-containing 4-[(4-methacryloyloxy)phenylazo]benzenesulfonic acid (MAPASA) as the functional monomer, 1,3,5-benzenetriol as a mimic template, and tetramethacryloyl triethylene tetramine (TTT) as the cross-linker. The MIHs show specific affinity to melamine with a binding constant of 3.20 × 10 4 M -1 in aqueous Tris-HCl buffer at pH 7.0. The density of the melamine-specific receptor sites in the MIHs material is 2.69 μmol g -1 MIHs. Upon alternate irradiation at 365 and 440 nm, the MIHs can quantitatively uptake and release melamine. The photoisomerization rate of MIHs is related to the concentration of melamine in the detecting solution, and therefore, a quick detection method for trace melamine is established. Analytical application of the MIHs to detect the melamine concentration in both milk and milk powder has been accomplished successfully with a simple pre-treatment of the samples. © 2012 The Royal Society of Chemistry.
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U2 - 10.1039/c2jm34522f
DO - 10.1039/c2jm34522f
M3 - RGC 21 - Publication in refereed journal
SN - 0959-9428
VL - 22
SP - 19812
EP - 19820
JO - Journal of Materials Chemistry
JF - Journal of Materials Chemistry
IS - 37
ER -