Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling

Jessica A.J. Liu; Ming-Hoi Wu; Carol H. Yan; Bolton K.H. Chau; Henry So; Alvis Ng; Alan Chan; Kathryn S.E. Cheah; James Briscoe; Martin Cheung

doi:10.1073/pnas.1211747110

Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling

Jessica A.J. Liu
, Ming-Hoi Wu
, Carol H. Yan
, Bolton K.H. Chau
, Henry So
, Alvis Ng
, Alan Chan
, Kathryn S.E. Cheah
, James Briscoe
, Martin Cheung

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

86 Citations (Scopus)

Abstract

Coordination of neural crest cell (NCC) induction and delamination is orchestrated by several transcription factors. Among these, Sry-related HMG box-9 (Sox9) and Snail2 have been implicated in both the induction of NCC identity and, together with phoshorylation, NCC delamination. How phosphorylation effects this function has not been clear. Here we show, in the developing chick neural tube, that phosphorylation of Sox9 on S64 and S181 facilitates its SUMOylation, and the phosphorylated forms of Sox9 are essential for trunk neural crest delamination. Both phosphorylation and to a lesser extent SUMOylation, of Sox9 are required to cooperate with Snail2 to promote delamination. Moreover, bone morphogenetic protein and canonical Wnt signaling induce phosphorylation of Sox9, thereby connecting extracellular signals with the delamination of NCCs. Together the data suggest a model in which extracellular signals initiate phosphorylation of Sox9 and its cooperation with Snail2 to induce NCC delamination.

Original language	English
Pages (from-to)	2882-2887
Journal	PNAS: Proceedings of the National Academy of Sciences of the United States of America
Volume	110
Issue number	8
DOIs	https://doi.org/10.1073/pnas.1211747110
Publication status	Published - 19 Feb 2013
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Basement membrane
In ovo electroporation

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10.1073/pnas.1211747110

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Liu, J. A. J., Wu, M.-H., Yan, C. H., Chau, B. K. H., So, H., Ng, A., Chan, A., Cheah, K. S. E., Briscoe, J., & Cheung, M. (2013). Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling. PNAS: Proceedings of the National Academy of Sciences of the United States of America, 110(8), 2882-2887. https://doi.org/10.1073/pnas.1211747110

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title = "Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling",

abstract = "Coordination of neural crest cell (NCC) induction and delamination is orchestrated by several transcription factors. Among these, Sry-related HMG box-9 (Sox9) and Snail2 have been implicated in both the induction of NCC identity and, together with phoshorylation, NCC delamination. How phosphorylation effects this function has not been clear. Here we show, in the developing chick neural tube, that phosphorylation of Sox9 on S64 and S181 facilitates its SUMOylation, and the phosphorylated forms of Sox9 are essential for trunk neural crest delamination. Both phosphorylation and to a lesser extent SUMOylation, of Sox9 are required to cooperate with Snail2 to promote delamination. Moreover, bone morphogenetic protein and canonical Wnt signaling induce phosphorylation of Sox9, thereby connecting extracellular signals with the delamination of NCCs. Together the data suggest a model in which extracellular signals initiate phosphorylation of Sox9 and its cooperation with Snail2 to induce NCC delamination.",

keywords = "Basement membrane, In ovo electroporation",

author = "Liu, \{Jessica A.J.\} and Ming-Hoi Wu and Yan, \{Carol H.\} and Chau, \{Bolton K.H.\} and Henry So and Alvis Ng and Alan Chan and Cheah, \{Kathryn S.E.\} and James Briscoe and Martin Cheung",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]. ",

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Liu, JAJ, Wu, M-H, Yan, CH, Chau, BKH, So, H, Ng, A, Chan, A, Cheah, KSE, Briscoe, J & Cheung, M 2013, 'Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling', PNAS: Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 8, pp. 2882-2887. https://doi.org/10.1073/pnas.1211747110

Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling. / Liu, Jessica A.J.; Wu, Ming-Hoi; Yan, Carol H. et al.
In: PNAS: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 8, 19.02.2013, p. 2882-2887.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling

AU - Liu, Jessica A.J.

AU - Wu, Ming-Hoi

AU - Yan, Carol H.

AU - Chau, Bolton K.H.

AU - So, Henry

AU - Ng, Alvis

AU - Chan, Alan

AU - Cheah, Kathryn S.E.

AU - Briscoe, James

AU - Cheung, Martin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2013/2/19

Y1 - 2013/2/19

N2 - Coordination of neural crest cell (NCC) induction and delamination is orchestrated by several transcription factors. Among these, Sry-related HMG box-9 (Sox9) and Snail2 have been implicated in both the induction of NCC identity and, together with phoshorylation, NCC delamination. How phosphorylation effects this function has not been clear. Here we show, in the developing chick neural tube, that phosphorylation of Sox9 on S64 and S181 facilitates its SUMOylation, and the phosphorylated forms of Sox9 are essential for trunk neural crest delamination. Both phosphorylation and to a lesser extent SUMOylation, of Sox9 are required to cooperate with Snail2 to promote delamination. Moreover, bone morphogenetic protein and canonical Wnt signaling induce phosphorylation of Sox9, thereby connecting extracellular signals with the delamination of NCCs. Together the data suggest a model in which extracellular signals initiate phosphorylation of Sox9 and its cooperation with Snail2 to induce NCC delamination.

AB - Coordination of neural crest cell (NCC) induction and delamination is orchestrated by several transcription factors. Among these, Sry-related HMG box-9 (Sox9) and Snail2 have been implicated in both the induction of NCC identity and, together with phoshorylation, NCC delamination. How phosphorylation effects this function has not been clear. Here we show, in the developing chick neural tube, that phosphorylation of Sox9 on S64 and S181 facilitates its SUMOylation, and the phosphorylated forms of Sox9 are essential for trunk neural crest delamination. Both phosphorylation and to a lesser extent SUMOylation, of Sox9 are required to cooperate with Snail2 to promote delamination. Moreover, bone morphogenetic protein and canonical Wnt signaling induce phosphorylation of Sox9, thereby connecting extracellular signals with the delamination of NCCs. Together the data suggest a model in which extracellular signals initiate phosphorylation of Sox9 and its cooperation with Snail2 to induce NCC delamination.

KW - Basement membrane

KW - In ovo electroporation

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U2 - 10.1073/pnas.1211747110

DO - 10.1073/pnas.1211747110

M3 - RGC 21 - Publication in refereed journal

C2 - 23382206

SN - 0027-8424

VL - 110

SP - 2882

EP - 2887

JO - PNAS: Proceedings of the National Academy of Sciences of the United States of America

JF - PNAS: Proceedings of the National Academy of Sciences of the United States of America

IS - 8

ER -

Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling

Abstract

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Research Keywords

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