Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling

Jessica A.J. Liu; Ming-Hoi Wu; Carol H. Yan; Bolton K.H. Chau; Henry So; Alvis Ng; Alan Chan; Kathryn S.E. Cheah; James Briscoe; Martin Cheung

doi:10.1073/pnas.1211747110

Author(s)

Ming-Hoi Wu
Carol H. Yan
Bolton K.H. Chau
Henry So
Alvis Ng
Alan Chan
Kathryn S.E. Cheah
James Briscoe
Martin Cheung

Detail(s)

Original language	English
Pages (from-to)	2882-2887
Journal / Publication	Proceedings of the National Academy of Sciences of the United States of America
Volume	110
Issue number	8
Publication status	Published - 19 Feb 2013
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1073/pnas.1211747110 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84874247173&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(8d550763-b2b5-40e9-90ab-93117c1e9e3b).html

Abstract

Coordination of neural crest cell (NCC) induction and delamination is orchestrated by several transcription factors. Among these, Sry-related HMG box-9 (Sox9) and Snail2 have been implicated in both the induction of NCC identity and, together with phoshorylation, NCC delamination. How phosphorylation effects this function has not been clear. Here we show, in the developing chick neural tube, that phosphorylation of Sox9 on S64 and S181 facilitates its SUMOylation, and the phosphorylated forms of Sox9 are essential for trunk neural crest delamination. Both phosphorylation and to a lesser extent SUMOylation, of Sox9 are required to cooperate with Snail2 to promote delamination. Moreover, bone morphogenetic protein and canonical Wnt signaling induce phosphorylation of Sox9, thereby connecting extracellular signals with the delamination of NCCs. Together the data suggest a model in which extracellular signals initiate phosphorylation of Sox9 and its cooperation with Snail2 to induce NCC delamination.

Research Area(s)

Basement membrane, In ovo electroporation

Bibliographic Note

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Citation Format(s)

[ RIS ] [ BibTeX ]

Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling. / Liu, Jessica A.J.; Wu, Ming-Hoi; Yan, Carol H. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 8, 19.02.2013, p. 2882-2887.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Liu, JAJ, Wu, M-H, Yan, CH, Chau, BKH, So, H, Ng, A, Chan, A, Cheah, KSE, Briscoe, J & Cheung, M 2013, 'Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 8, pp. 2882-2887. https://doi.org/10.1073/pnas.1211747110

Liu, J. A. J., Wu, M.-H., Yan, C. H., Chau, B. K. H., So, H., Ng, A., Chan, A., Cheah, K. S. E., Briscoe, J., & Cheung, M. (2013). Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America, 110(8), 2882-2887. https://doi.org/10.1073/pnas.1211747110

Liu JAJ, Wu MH, Yan CH, Chau BKH, So H, Ng A et al. Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America. 2013 Feb 19;110(8):2882-2887. doi: 10.1073/pnas.1211747110

Liu, Jessica A.J. ; Wu, Ming-Hoi ; Yan, Carol H. et al. / Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 8. pp. 2882-2887.