β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell-material interface compared to α-phase poly(vinylidene fluoride) films

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Y.K.A. Low
  • X. Zou
  • Y.M. Fang
  • J.L. Wang
  • W.S. Lin
  • K.W. Ng

Detail(s)

Original languageEnglish
Pages (from-to)345-353
Journal / PublicationMaterials Science and Engineering C
Volume34
Online published2 Oct 2013
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Abstract

The piezoelectric response from β-phase poly(vinylidene fluoride) (PVDF) can potentially be exploited for biomedical application. We hypothesized that α and β-phase PVDF exert direct but different influence on cellular behavior. α- and β-phase PVDF films were synthesized through solution casting and characterized with FT-IR, XRD, AFM and PFM to ensure successful fabrication of α and β-phase PVDF films. Cellular evaluation with L929 mouse fibroblasts over one-week was conducted with AlamarBlue® metabolic assay and PicoGreen® proliferation assay. Immunostaining of fibronectin investigated the extent and distribution of extracellular matrix deposition. Image saliency analysis quantified differences in cellular distribution on the PVDF films. Our results showed that β-phase PVDF films with the largest area expressing piezoelectric effect elicited highest cell metabolic activity at day 3 of culture. Increased fibronectin adsorption towards the cell-material interface was shown on β-phase PVDF films. Image saliency analysis showed that fibroblasts on β-phase PVDF films were more homogeneously distributed than on α-phase PVDF films. Taken collectively, the different molecular packing of α and β-phase PVDF resulted in differing physical properties of films, which in turn induced differences in cellular behaviors. Further analysis of how α and β-phase PVDF may evoke specific cellular behavior to suit particular application will be intriguing. © 2013 Elsevier B.V.

Research Area(s)

  • Biomaterial, Cell-material interaction, Poly(vinylidene fluoride), Polymorphism

Citation Format(s)

β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell-material interface compared to α-phase poly(vinylidene fluoride) films. / Low, Y.K.A.; Zou, X.; Fang, Y.M. et al.
In: Materials Science and Engineering C, Vol. 34, 01.01.2014, p. 345-353.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review