Peptide-Au Cluster Probe : Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

19 Scopus Citations
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Author(s)

  • Lina Zhao
  • Lingna Zheng
  • Fuping Gao
  • Liang Gao
  • Ru Liu
  • Yaling Wang
  • Xueyun Gao

Detail(s)

Original languageEnglish
Pages (from-to)276-282
Journal / PublicationACS Omega
Volume2
Issue number1
Online published27 Jan 2017
Publication statusPublished - 31 Jan 2017
Externally publishedYes

Link(s)

Abstract

Alterations in protein (e.g., biomarkers) expression levels have a significant correlation with tumor development and prognosis; therefore, it is desired to develop precise methods to differentiate the expression level of proteins in tumor cell lines, especially at the single-cell level. Here, we report a precise and versatile approach of quantifying the protein expression levels of three tumor cell lines in situ using a peptide-Au cluster probe. The probe (Au5Peptide3) consists of a peptide with a specific cell membrane epidermal growth factor receptor (EGFR) targeting ability and an Au cluster for both cell membrane EGFR imaging using confocal microscopy and cell membrane EGFR counting by laser ablation inductively coupled plasma mass spectrometry. Utilizing the peptide-Au cluster probe, we successfully quantify the EGFR expression levels of SMMC-7721, KB, and HeLa cells at a single-cell level and differentiate the EGFR expression levels among these cell lines. The peptide-Au cluster probe, with the ability to differentiate the protein expression level of different cell lines, shows exceptional promise for providing reliable predictive and prognostic information of tumors at a single-cell level.

Research Area(s)

Citation Format(s)

Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level. / Zhai, Jiao; Zhao, Lina; Zheng, Lingna et al.
In: ACS Omega, Vol. 2, No. 1, 31.01.2017, p. 276-282.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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