Peptide microarray for high-throughput determination of phosphatase specificity and biology

Hongyan Sun, Candy H. S. Lu, Mahesh Uttamchandani, Yun Xia, Yih-Cherng Liou, Shao Q. Yao

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

55 Citations (Scopus)

Abstract

Identifying phosphatase substrates: A peptide microarray has been developed for the high-throughput study of Ser/Thr phosphatases. Putative peptide substrates, upon immobilization onto a glass slide, could be used to obtain kinetic information and identify the substrate preferences of a Ser/Thr phosphatase (see schematic representation); with this information, new biology of the enzyme could be discovered. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
Original languageEnglish
Pages (from-to)1698-1702
JournalAngewandte Chemie - International Edition
Volume47
Issue number9
DOIs
Publication statusPublished - 15 Feb 2008
Externally publishedYes

Research Keywords

  • Microarrays
  • Peptides
  • Phosphatases
  • Phosphorylation
  • Substrate specificity

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