TY - JOUR
T1 - Peptide-Bridged Assembly of Hybrid Nanomaterial and Its Application for Caspase-3 Detection
AU - Shi, Yupeng
AU - Yi, Changqing
AU - Zhang, Zhaomin
AU - Zhang, Heng
AU - Li, Meijin
AU - Yang, Mengsu
AU - Jiang, Qing
PY - 2013/7/24
Y1 - 2013/7/24
N2 - Recent developments in the rational design and the controlled assembly of nanoscale building blocks have resulted in functional devices such as nano-optoelectronics, novel contrast probes for molecular imaging, and nanosensors. In the present study, we designed and synthesized a hybrid nanomaterial consisting of [Ru(bpy)3]2+-encapsulated silica nanoparticles (SiNPs) and gold nanoparticles (AuNPs) through peptide-bridged assembly in a controllable way. A peptide that contains recognition sequence DEVD specific for active caspase-3 cleavage was employed to bring SiNPs and AuNPs into close proximity through specific molecular recognition. A FRET system with SiNPs as energy donors and AuNPs as energy acceptors has been thus developed and applied for caspase-3 detection. A change in distance between the two building blocks resulted in a change in FRET efficiency, causing a ratiometric change in emission. Caspase-3 triggers the cleavage of the peptide links between the two nanoparticles and releases AuNPs from the nanohybrids, inducing the activation of SiNPs to the "ON" state. The fluorescence turn-on response is specific to caspase-3 and allows the detection of caspase-3 as low as 0.05 U mL-1 (∼6 pM). © 2013 American Chemical Society.
AB - Recent developments in the rational design and the controlled assembly of nanoscale building blocks have resulted in functional devices such as nano-optoelectronics, novel contrast probes for molecular imaging, and nanosensors. In the present study, we designed and synthesized a hybrid nanomaterial consisting of [Ru(bpy)3]2+-encapsulated silica nanoparticles (SiNPs) and gold nanoparticles (AuNPs) through peptide-bridged assembly in a controllable way. A peptide that contains recognition sequence DEVD specific for active caspase-3 cleavage was employed to bring SiNPs and AuNPs into close proximity through specific molecular recognition. A FRET system with SiNPs as energy donors and AuNPs as energy acceptors has been thus developed and applied for caspase-3 detection. A change in distance between the two building blocks resulted in a change in FRET efficiency, causing a ratiometric change in emission. Caspase-3 triggers the cleavage of the peptide links between the two nanoparticles and releases AuNPs from the nanohybrids, inducing the activation of SiNPs to the "ON" state. The fluorescence turn-on response is specific to caspase-3 and allows the detection of caspase-3 as low as 0.05 U mL-1 (∼6 pM). © 2013 American Chemical Society.
KW - caspase-3
KW - fluorescence resonance energy transfer
KW - gold nanoparticles
KW - hybrid nanomaterials
KW - silica nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84880814639&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84880814639&origin=recordpage
U2 - 10.1021/am401935y
DO - 10.1021/am401935y
M3 - RGC 21 - Publication in refereed journal
C2 - 23806127
SN - 1944-8244
VL - 5
SP - 6494
EP - 6501
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 14
ER -