Pentobarbital modulates transmitter effects on mouse spinal neurones grown in tissue culture

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Original languageEnglish
Pages (from-to)703-705
Journal / PublicationNature
Issue number5502
Publication statusPublished - 1975
Externally publishedYes


GENERAL anaesthetics depress postsynaptic excitatory transmission in the vertebrate central and peripheral nervous systems1-4 although preserving or prolonging both presynaptic5-6 and postsynaptic inhibition7-10. The mechanisms underlying these cellular events and their precise relationship with the phenomenon of general anaesthesia in mammals have not been elucidated. We have used various invertebrate preparations to show that pentobarbital and other general anaesthetics operate at a postsynaptic level to depress Na+-dependent postsynaptic excitation without affecting either Cl-- or K+-dependent postsynaptic inhibition11,12. Here, using intracellular recording from mouse spinal neurones grown in tissue culture, we show that pentobarbital depresses glutamate excitation and prolongs γ-aminobutyric acid (GABA) inhibition in most cells, through a postsynaptic mechanism. © 1975 Nature Publishing Group.