PEGylated dihydromyricetin-loaded nanoliposomes coated with tea saponin inhibit bacterial oxidative respiration and energy metabolism

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Fan Luo
  • Dandan Zeng
  • Renxiang Chen
  • Ayesha Zafar
  • Ling Weng
  • Yubo Tian
  • Murtaza Hasan
  • Xugang Shu

Detail(s)

Original languageEnglish
Pages (from-to)9007-9017
Journal / PublicationFood & Function
Volume12
Issue number19
Online published12 Aug 2021
Publication statusPublished - 7 Oct 2021

Abstract

The biofilms produced by the aggregation of bacterial colonies are among the major obstacles of host immune system monitoring and antimicrobial treatment. Herein, we report PEGylated dihydromyricetin-loaded liposomes coated with tea saponin grafted on chitosan (TS/CTS@DMY-lips) as an efficient cationic antibacterial agent against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), which is supported by their deep penetration into bacterial biofilms and broad pH-stable release performance of dihydromyricetin (DMY). The successful construction of the drug delivery system relied on tea saponin grafted on chitosan (TS/CTS) via formatted ester bonds or amido bonds as a polyelectrolyte layer of PEGylated dihydromyricetin-loaded liposomes (DMY lips), which achieved controlled release of DMY in weak acidic and neutral physiological environments. The micromorphology of TS/CTS@DMY-lips was observed to resemble dendritic cells with an average size of 266.49 nm, and they had excellent encapsulation efficiency (41.93%), water-solubility and stability in aqueous solution. Besides, TS/CTS@DMY-lips displayed effective destruction of bacterial energy metabolism and cytoplasmic membranes, resulting in the deformation of the cell wall and leaking of cytoplasmic constituents. Compared to free DMY, DMY lips and chitosan-coated dihydromyricetin liposomes (CTS@DMY-lips), TS/CTS@DMY-lips has more thorough killing activity against E. coli and S. aureus, which is related to its excellent sustained release performance of DMY under the protection of the TS/CTS coating.

Research Area(s)

  • IN-VITRO RELEASE, ANTIBACTERIAL MECHANISM, CHITOSAN, LIPOSOMES, STABILITY, FOOD

Citation Format(s)

PEGylated dihydromyricetin-loaded nanoliposomes coated with tea saponin inhibit bacterial oxidative respiration and energy metabolism. / Luo, Fan; Zeng, Dandan; Chen, Renxiang; Zafar, Ayesha; Weng, Ling; Wang, Wenxiong; Tian, Yubo; Hasan, Murtaza; Shu, Xugang.

In: Food & Function, Vol. 12, No. 19, 07.10.2021, p. 9007-9017.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review