PEG-PtS2 nanosheet-based fluorescence biosensor for label-free human papillomavirus genotyping

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Wenxian Zhang
  • Shenchang Li
  • Xiuping Li
  • Man Liu
  • Tianyu Cui
  • Huayang Fu
  • Wenying Zhong
  • Bo Xu
  • Wanqing Yue

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number408
Journal / PublicationMicrochimica Acta
Volume187
Issue number7
Online published29 Jun 2020
Publication statusPublished - Jul 2020

Abstract

A simple and efficient ultrasonication-assisted liquid exfoliation method is proposed to produce PtS2 nanosheets on a large scale and improve their dispersion in aqueous solution by surface polyethylene glycol modification. The interaction of polyethylene glycol-modified PtS2 (PEG-PtS2) nanosheets with fluorescent labeled DNA and the fluorescence quenching mechanism using FAM-labeled hpv16e6 gene fragment as a probe was investigated. The excitation and emission wavelengths were 468 and 517 nm, respectively. The fluorescence quenching mechanism of PEG-PtS2 nanosheets for double-stranded DNA (dsDNA) might stem from the static quenching effect. Based on the difference in fluorescence quenching capability of PEG-PtS2 nanosheets in fluorescent probe tagged single-stranded DNA (ssDNA) and dsDNA, a mix-and-detect method was proposed for determination of DNA. Without the need for probe immobilization and tedious washing steps, the genotyping of human papillomavirus (HPV) was easily achieved. The limit of detection was calculated to 0.44 nM, showing a good linear range within 0.05–10 nM. We believe this biosensor provides opportunities to develop a simple and low-cost strategy for molecular diagnostics.

Research Area(s)

  • DNA fluorescence biosensor, Platinum disulfide nanosheets, Polyethylene glycol modification, Ultrasonication-assisted liquid exfoliation method

Citation Format(s)

PEG-PtS2 nanosheet-based fluorescence biosensor for label-free human papillomavirus genotyping. / Zhang, Wenxian; Li, Shenchang; Li, Xiuping; Liu, Man; Cui, Tianyu; Fu, Huayang; Yang, Mengsu; Zhong, Wenying; Xu, Bo; Yue, Wanqing.

In: Microchimica Acta, Vol. 187, No. 7, 408, 07.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review