PCR and Molecular Detection for Differentiating Vibrio Species

O. A. E. SPARAGANO; P. A. W. ROBERTSON; I. PURDOM; J. MCINNES; Y. LI; D.-H. YU; Z.-J. DU; H.-S. XU; B. AUSTIN

doi:10.1111/j.1749-6632.2002.tb04351.x

PCR and Molecular Detection for Differentiating Vibrio Species

O. A. E. SPARAGANO
, P. A. W. ROBERTSON
, I. PURDOM
, J. MCINNES
, Y. LI
, D.-H. YU
, Z.-J. DU
, H.-S. XU
, B. AUSTIN

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

13 Citations (Scopus)

Abstract

Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

Original language	English
Pages (from-to)	60-65
Journal	Annals of the New York Academy of Sciences
Volume	969
Issue number	1
DOIs	https://doi.org/10.1111/j.1749-6632.2002.tb04351.x
Publication status	Published - Oct 2002
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Research Keywords

16S PCR reactions
23S PCR reactions
Aquaculture
Vibrio species
Vibriosis

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title = "PCR and Molecular Detection for Differentiating Vibrio Species",

abstract = "Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.",

keywords = "16S PCR reactions, 23S PCR reactions, Aquaculture, Vibrio species, Vibriosis",

author = "SPARAGANO, \{O. A. E.\} and ROBERTSON, \{P. A. W.\} and I. PURDOM and J. MCINNES and Y. LI and D.-H. YU and Z.-J. DU and H.-S. XU and B. AUSTIN",

year = "2002",

month = oct,

doi = "10.1111/j.1749-6632.2002.tb04351.x",

language = "English",

volume = "969",

pages = "60--65",

journal = "Annals of the New York Academy of Sciences",

issn = "0077-8923",

publisher = "John Wiley \& Sons",

number = "1",

}

TY - JOUR

T1 - PCR and Molecular Detection for Differentiating Vibrio Species

AU - SPARAGANO, O. A. E.

AU - ROBERTSON, P. A. W.

AU - PURDOM, I.

AU - MCINNES, J.

AU - LI, Y.

AU - YU, D.-H.

AU - DU, Z.-J.

AU - XU, H.-S.

AU - AUSTIN, B.

PY - 2002/10

Y1 - 2002/10

N2 - Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

AB - Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

KW - 16S PCR reactions

KW - 23S PCR reactions

KW - Aquaculture

KW - Vibrio species

KW - Vibriosis

UR - https://www.scopus.com/pages/publications/0036411702

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-0036411702&origin=recordpage

U2 - 10.1111/j.1749-6632.2002.tb04351.x

DO - 10.1111/j.1749-6632.2002.tb04351.x

M3 - RGC 21 - Publication in refereed journal

C2 - 12381564

SN - 0077-8923

VL - 969

SP - 60

EP - 65

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

IS - 1

ER -

PCR and Molecular Detection for Differentiating Vibrio Species

Abstract

UN SDGs

Research Keywords

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