PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • ANDREW S. J. MIKOSZA
  • TOM LA
  • C. JOSEPHINE BROOKE
  • CHRISTIAN F. LINDBOE
  • PETER. B WARD
  • RALF G. HEINE
  • JOHN G. GUCCION
  • W. BASTIAAN DE BOER

Detail(s)

Original languageEnglish
Pages (from-to)2093-2098
Journal / PublicationJournal of Clinical Microbiology
Volume37
Issue number6
Publication statusPublished - Jun 1999
Externally publishedYes

Abstract

PCR procedures amplifying portions of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Serpulina pilosicoli were applied to DNA extracted from paraffin-embedded human colonic or rectal tissues from 30 Norwegian, Australian, and U.S. patients, 16 of whom had histologic evidence of intestinal spirochetosis (IS). B. aalborgi-specific sequences were identified by PCR in 10 of the IS patients (62.5%) but none of the others, while S. pilosicoli sequences were not detected in tissues from any patient. Direct sequencing of products from three of the positive samples provided further confirmation of the presence of B. aalborgi. B. aalborgi may be a more common cause of intestinal spirochetosis than has been previously thought.

Citation Format(s)

PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis. / MIKOSZA, ANDREW S. J. ; LA, TOM; BROOKE, C. JOSEPHINE ; LINDBOE, CHRISTIAN F. ; WARD, PETER. B; HEINE, RALF G. ; GUCCION, JOHN G. ; DE BOER, W. BASTIAAN ; HAMPSON, DAVID J.

In: Journal of Clinical Microbiology, Vol. 37, No. 6, 06.1999, p. 2093-2098.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review