Organic Semiconducting Polymer Nanoparticles for Photoacoustic Labeling and Tracking of Stem Cells in the Second Near-Infrared Window

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chao Yin
  • Boguang Yang
  • Sien Lin
  • Jiawei Huang
  • Pengchao Zhao
  • Siu Hong Dexter Wong
  • Kunyu Zhang
  • Xiaoyu Chen
  • Gang Li
  • Xiaohua Jiang
  • Jianping Huang
  • Kanyi Pu
  • Liming Bian

Detail(s)

Original languageEnglish
Pages (from-to)12201-12211
Journal / PublicationACS Nano
Volume12
Issue number12
Online published15 Nov 2018
Publication statusPublished - 26 Dec 2018

Abstract

Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window hamper the applications of PA imaging in living subjects. Herein, we report the design and synthesis of a second near-infrared (NIR-II) absorptive organic semiconducting polymer (OSP)-based nanoprobe (OSPN+) for PA imaging and tracking of stem cells. Comparison studies in biological tissue show that NIR-II light excited PA imaging of the OSPN+ has significantly higher signal-to-noise ratio than NIR-I light excited PA imaging, thereby demonstrating the superiority of the OSPN+ for deep tissue imaging. With good biocompatibility, appropriate size, and optimized surface property, the OSPN+ shows enhanced cellular uptake for highly efficient PA labeling of stem cells. In vivo investigations reveal significant NIR-II PA contrast enhancement of the transplanted OSPN+-labeled human mesenchymal stem cells by 40.6- and 21.7-fold in subcutaneous and brain imaging, respectively, compared with unlabeled cases. Our work demonstrates a class of OSP-based nanomaterials for NIR-II PA stem cell imaging to facilitate a better understanding and evaluation of stem cell-based therapies.

Research Area(s)

  • nanoparticle, organic semiconducting polymer, photoacoustic imaging, second near-infrared absorption, stem cell

Citation Format(s)

Organic Semiconducting Polymer Nanoparticles for Photoacoustic Labeling and Tracking of Stem Cells in the Second Near-Infrared Window. / Yin, Chao; Wen, Guohua; Liu, Chao; Yang, Boguang; Lin, Sien; Huang, Jiawei; Zhao, Pengchao; Wong, Siu Hong Dexter; Zhang, Kunyu; Chen, Xiaoyu; Li, Gang; Jiang, Xiaohua; Huang, Jianping; Pu, Kanyi; Wang, Lidai; Bian, Liming.

In: ACS Nano, Vol. 12, No. 12, 26.12.2018, p. 12201-12211.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review