Optimized protocols for ChIP-seq and deletion mutant construction in Pseudomonas syringae

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Detail(s)

Original languageEnglish
Article number100776
Journal / PublicationSTAR Protocols
Volume2
Issue number3
Online published25 Aug 2021
Publication statusPublished - 17 Sep 2021

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Abstract

Chromatin immunoprecipitation sequencing (ChIP-seq) is an efficient technique to identify the binding sites of transcription factors (TFs) in both eukaryotes and prokaryotes. However, its application in bacteria is very heterogeneous. In this protocol, we optimized the methods of ChIP-seq that can be widely applied to plant pathogens. We used homologous recombination to construct pK18mobsacB-Psph plasmid instead of restriction site ligation and replaced transconjugation with electroporation transformation in Pseudomonas syringae deletion mutant construction, which is more efficient and faster than previous methods. For complete details on the use and execution of this protocol, please refer to Shao et al. (2021).

Research Area(s)

  • ChIPseq, Genetics, Microbiology, Molecular Biology, Sequencing

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