Optical-resolution photoacoustic microscopy with ultrafast dual-wavelength excitation

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Yingying Zhou
  • Chengbo Liu
  • Puxiang Lai

Detail(s)

Original languageEnglish
Article numbere201960229
Journal / PublicationJournal of Biophotonics
Volume13
Issue number6
Online published12 Feb 2020
Publication statusPublished - Jun 2020

Abstract

Fast functional and molecular photoacoustic microscopy requires pulsed laser excitations at multiple wavelengths with enough pulse energy and short wavelength-switching time. Recent development of stimulated Raman scattering in optical fiber offers a low-cost laser source for multiwavelength photoacoustic imaging. In this approach, long fibers temporally separate different wavelengths via optical delay. The time delay between adjacent wavelengths may eventually limits the highest A-line rate. In addition, a long-time delay in fiber may limit the highest pulse energy, leading to poor image quality. In order to achieve high pulse energy and ultrafast dual-wavelength excitation, we present optical-resolution photoacoustic microscopy with ultrafast dual-wavelength excitation and a signal separation method. The signal separation method is validated in numerical simulation and phantom experiments. We show that when two photoacoustic signals are partially overlapped with a 50-ns delay, they can be recovered with 98% accuracy. We apply this ultrafast dual-wavelength excitation technique to in vivo OR-PAM. Results demonstrate that A-lines at two wavelengths can be successfully separated, and sO2 values can be reliably computed from the separated data. The ultrafast dual-wavelength excitation enables fast functional photoacoustic microscopy with negligible misalignment among different wavelengths and high pulse energy, which is important for in vivo imaging of microvascular dynamics.

Research Area(s)

  • fast dual-wavelength excitation, functional photoacoustic imaging, multiwavelength, signal separation, OXYGEN-SATURATION, FIBER-LASER, VIVO

Citation Format(s)

Optical-resolution photoacoustic microscopy with ultrafast dual-wavelength excitation. / Zhou, Yingying; Liang, Siyi; Li, Mingsheng et al.
In: Journal of Biophotonics, Vol. 13, No. 6, e201960229, 06.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review