Optical sectioning fluorescence microscopy using variable metalens

Cheng Hung Chu; Sunil Vyas; Hsin Yu Kuo; Yu Hsin Chia; Mu Ku Chen; Yi-You Huang; Din Ping Tsai; Yuan Luo

doi:10.1117/12.3007925

Optical sectioning fluorescence microscopy using variable metalens

Cheng Hung Chu
, Sunil Vyas
, Hsin Yu Kuo
, Yu Hsin Chia
, Mu Ku Chen
, Yi-You Huang
, Din Ping Tsai
, Yuan Luo

Department of Electrical Engineering

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Fluorescence microscopy is a common method to observe the structural features of biological samples. Conventional tunable lenses are usually used to axially scan the volumetric samples for obtaining fluorescence optical sectioning images. However, they suffer from spherical aberration and distortions. In this work, we propose a compact imaging system with optical sectioning capability based on Moiré metalens which consists of two complementary phase metasurfaces to perform fluorescence bio-imaging applications in visible regions. The focal length of system can be tuned from 10 mm to 125 mm at the wavelength of 532 nm by changing mutual angles between two metasurfaces. In addition, the speckle illumination HiLo microscope is used to reduce the effect of out-of-focus light scattering. To demonstrate the optical sectioning capability of our system, the labeled beads as well as ex vivo mice intestine tissue samples are imaged. The presented design of varifocal metalens is anticipated to realize important applications in fluorescence microscopy and endoscopy. © 2023 SPIE.

Original language	English
Title of host publication	Biomedical Imaging and Sensing Conference
Editors	Toyohiko Yatagai
Publisher	SPIE
ISBN (Electronic)	9781510663442
ISBN (Print)	9781510663435
DOIs	https://doi.org/10.1117/12.3007925
Publication status	Published - 2023
Event	Optics and Photonics International Congress 2023 (OPIC 2023) - Pacifico Yokohama (hybrid), Yokohama, Japan Duration: 17 Apr 2023 → 21 Apr 2023 https://opicon.jp/opic2023/about/aboutopic/

Publication series

Name	Proceedings of SPIE
Volume	12608
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Optics and Photonics International Congress 2023 (OPIC 2023)
Place	Japan
City	Yokohama
Period	17/04/23 → 21/04/23
Internet address	https://opicon.jp/opic2023/about/aboutopic/

Bibliographical note

Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).

Research Keywords

fluorescence microscopy
metasurface
optical sectioning

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10.1117/12.3007925

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Cite this

@inproceedings{2573632b875f4f75bb589e9859637f3e,

title = "Optical sectioning fluorescence microscopy using variable metalens",

abstract = "Fluorescence microscopy is a common method to observe the structural features of biological samples. Conventional tunable lenses are usually used to axially scan the volumetric samples for obtaining fluorescence optical sectioning images. However, they suffer from spherical aberration and distortions. In this work, we propose a compact imaging system with optical sectioning capability based on Moir{\'e} metalens which consists of two complementary phase metasurfaces to perform fluorescence bio-imaging applications in visible regions. The focal length of system can be tuned from 10 mm to 125 mm at the wavelength of 532 nm by changing mutual angles between two metasurfaces. In addition, the speckle illumination HiLo microscope is used to reduce the effect of out-of-focus light scattering. To demonstrate the optical sectioning capability of our system, the labeled beads as well as ex vivo mice intestine tissue samples are imaged. The presented design of varifocal metalens is anticipated to realize important applications in fluorescence microscopy and endoscopy. {\textcopyright} 2023 SPIE.",

keywords = "fluorescence microscopy, metasurface, optical sectioning",

author = "Chu, \{Cheng Hung\} and Sunil Vyas and Kuo, \{Hsin Yu\} and Chia, \{Yu Hsin\} and Chen, \{Mu Ku\} and Yi-You Huang and Tsai, \{Din Ping\} and Yuan Luo",

note = "Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).; Optics and Photonics International Congress 2023 (OPIC 2023) ; Conference date: 17-04-2023 Through 21-04-2023",

year = "2023",

doi = "10.1117/12.3007925",

language = "English",

isbn = "9781510663435",

series = "Proceedings of SPIE",

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editor = "Toyohiko Yatagai",

booktitle = "Biomedical Imaging and Sensing Conference",

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url = "https://opicon.jp/opic2023/about/aboutopic/",

}

Chu, CH, Vyas, S, Kuo, HY, Chia, YH, Chen, MK, Huang, Y-Y, Tsai, DP & Luo, Y 2023, Optical sectioning fluorescence microscopy using variable metalens. in T Yatagai (ed.), Biomedical Imaging and Sensing Conference., 1260812, Proceedings of SPIE, vol. 12608, SPIE, Optics and Photonics International Congress 2023 (OPIC 2023), Yokohama, Japan, 17/04/23. https://doi.org/10.1117/12.3007925

Optical sectioning fluorescence microscopy using variable metalens. / Chu, Cheng Hung; Vyas, Sunil; Kuo, Hsin Yu et al.
Biomedical Imaging and Sensing Conference. ed. / Toyohiko Yatagai. SPIE, 2023. 1260812 (Proceedings of SPIE; Vol. 12608).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Optical sectioning fluorescence microscopy using variable metalens

AU - Chu, Cheng Hung

AU - Vyas, Sunil

AU - Kuo, Hsin Yu

AU - Chia, Yu Hsin

AU - Chen, Mu Ku

AU - Huang, Yi-You

AU - Tsai, Din Ping

AU - Luo, Yuan

N1 - Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).

PY - 2023

Y1 - 2023

N2 - Fluorescence microscopy is a common method to observe the structural features of biological samples. Conventional tunable lenses are usually used to axially scan the volumetric samples for obtaining fluorescence optical sectioning images. However, they suffer from spherical aberration and distortions. In this work, we propose a compact imaging system with optical sectioning capability based on Moiré metalens which consists of two complementary phase metasurfaces to perform fluorescence bio-imaging applications in visible regions. The focal length of system can be tuned from 10 mm to 125 mm at the wavelength of 532 nm by changing mutual angles between two metasurfaces. In addition, the speckle illumination HiLo microscope is used to reduce the effect of out-of-focus light scattering. To demonstrate the optical sectioning capability of our system, the labeled beads as well as ex vivo mice intestine tissue samples are imaged. The presented design of varifocal metalens is anticipated to realize important applications in fluorescence microscopy and endoscopy. © 2023 SPIE.

AB - Fluorescence microscopy is a common method to observe the structural features of biological samples. Conventional tunable lenses are usually used to axially scan the volumetric samples for obtaining fluorescence optical sectioning images. However, they suffer from spherical aberration and distortions. In this work, we propose a compact imaging system with optical sectioning capability based on Moiré metalens which consists of two complementary phase metasurfaces to perform fluorescence bio-imaging applications in visible regions. The focal length of system can be tuned from 10 mm to 125 mm at the wavelength of 532 nm by changing mutual angles between two metasurfaces. In addition, the speckle illumination HiLo microscope is used to reduce the effect of out-of-focus light scattering. To demonstrate the optical sectioning capability of our system, the labeled beads as well as ex vivo mice intestine tissue samples are imaged. The presented design of varifocal metalens is anticipated to realize important applications in fluorescence microscopy and endoscopy. © 2023 SPIE.

KW - fluorescence microscopy

KW - metasurface

KW - optical sectioning

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UR - https://www.scopus.com/pages/publications/85176093188#tab=impact

U2 - 10.1117/12.3007925

DO - 10.1117/12.3007925

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510663435

T3 - Proceedings of SPIE

BT - Biomedical Imaging and Sensing Conference

A2 - Yatagai, Toyohiko

PB - SPIE

T2 - Optics and Photonics International Congress 2023 (OPIC 2023)

Y2 - 17 April 2023 through 21 April 2023

ER -

Optical sectioning fluorescence microscopy using variable metalens

Abstract

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Bibliographical note

Research Keywords

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