On-site quantitation of xanthine in fish and serum using a smartphone-based spectrophotometer integrated with a dual-readout nanosensing assay
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
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Article number | 137107 |
Journal / Publication | Food Chemistry |
Volume | 431 |
Online published | 6 Aug 2023 |
Publication status | Published - 15 Jan 2024 |
Link(s)
Abstract
Rapid and quantitative biochemical analysis at points-of-need is imperative for food safety inspection. This work reports on: 1) a stand-alone smartphone-based “two-in-one” spectrophotometer (the SAFS) installed with a self-developed application (the SAFS-App) which can precisely collect both absorption spectra and fluorescence spectra in a reproducible manner within 5 s; and 2) a straightforward protocol for xanthine detection using fluorescent carbon nanodots and silver nanoparticles. The assay performed with the SAFS demonstrates high specificity towards xanthine, and a linear range of 1–60 μM with LODs of 0.38 and 0.58 μM for colorimetric and fluorometric readouts, respectively. The reliability and robustness of the SAFS are validated by on-site quantitation of xanthine in fish and serum samples, with comparable accuracy to HPLC method. More importantly, the SAFS presents itself as an appealing device which is accessible to everyone through the Internet of Things and can be tailored for diverse point-of-care testing applications. © 2023 Elsevier Ltd.
Research Area(s)
- Carbon nanodots, Inner filter effect, Portable device, Silver nanoparticle, Xanthine
Citation Format(s)
On-site quantitation of xanthine in fish and serum using a smartphone-based spectrophotometer integrated with a dual-readout nanosensing assay. / Xu, Ningxia; Xiao, Meng; Yu, Zipei et al.
In: Food Chemistry, Vol. 431, 137107, 15.01.2024.
In: Food Chemistry, Vol. 431, 137107, 15.01.2024.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review