On-Site Melanoma Diagnosis Utilizing a Swellable Microneedle-Assisted Skin Interstitial Fluid Sampling and a Microfluidic Particle Dam for Visual Quantification of S100A1
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Related Research Unit(s)
Detail(s)
Original language | English |
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Article number | 2306188 |
Journal / Publication | Advanced Science |
Volume | 11 |
Issue number | 16 |
Online published | 28 Feb 2024 |
Publication status | Published - 24 Apr 2024 |
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DOI | DOI |
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Attachment(s) | Documents
Publisher's Copyright Statement
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85186231902&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(d57c7085-f461-4338-afc2-16e3656292ae).html |
Abstract
Malignant melanoma (MM) is the most aggressive form of skin cancer. The delay in treatment will induce metastasis, resulting in a poor prognosis and even death. Here, a two-step strategy for on-site diagnosis of MM is developed based on the extraction and direct visual quantification of S100A1, a biomarker for melanoma. First, a swellable microneedle is utilized to extract S100A1 in skin interstitial fluid (ISF) with minimal invasion. After elution, antibody-conjugated magnetic microparticles (MMPs) and polystyrene microparticles (PMPs) are introduced. A high expression level of S100A1 gives rise to a robust binding between MMPs and PMPs and reduces the number of free PMPs. By loading the reacted solution into the device with a microfluidic particle dam, the quantity of free PMPs after magnetic separation is displayed with their accumulation length inversely proportional to S100A1 levels. A limit of detection of 18.7 ng mL−1 for S100A1 is achieved. The animal experiment indicates that ISF-based S100A1 quantification using the proposed strategy exhibits a significantly higher sensitivity compared with conventional serum-based detection. In addition, the result is highly comparable with the gold standard enzyme-linked immunosorbent assay based on Lin's concordance correlation coefficient, suggesting the high practicality for routine monitoring of melanoma. © 2024 The Authors. Advanced Science published by Wiley-VCH GmbH.
Research Area(s)
- interstitial fluid, malignant melanoma, microfluidics, S100A1, swellable microneedle
Citation Format(s)
In: Advanced Science, Vol. 11, No. 16, 2306188, 24.04.2024.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review