One-pot dual-labeling of a protein by two chemoselective reactions

Long Yi; Hongyan Sun; Aymelt Itzen; Gemma Triola; Herbert Waldmann; Roger S. Goody; Yao-Wen Wu

doi:10.1002/anie.201100840

Author(s)

Long Yi
Aymelt Itzen
Gemma Triola
Herbert Waldmann
Roger S. Goody
Yao-Wen Wu

Detail(s)

Original language	English
Pages (from-to)	8287-8290
Journal / Publication	Angewandte Chemie - International Edition
Volume	50
Issue number	36
Publication status	Published - 29 Aug 2011
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1002/anie.201100840 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-80052077967&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(70e9be28-3280-4393-97d4-f0fac0087c2d).html

Abstract

Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Research Area(s)

chemoselective reactions, fluorescent labeling, native chemical ligation, oxime ligation, site-specific modification

Citation Format(s)

[ RIS ] [ BibTeX ]

One-pot dual-labeling of a protein by two chemoselective reactions. / Yi, Long; Sun, Hongyan; Itzen, Aymelt et al.

In: Angewandte Chemie - International Edition, Vol. 50, No. 36, 29.08.2011, p. 8287-8290.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Yi, L, Sun, H, Itzen, A, Triola, G, Waldmann, H, Goody, RS & Wu, Y-W 2011, 'One-pot dual-labeling of a protein by two chemoselective reactions', Angewandte Chemie - International Edition, vol. 50, no. 36, pp. 8287-8290. https://doi.org/10.1002/anie.201100840

Yi, L., Sun, H., Itzen, A., Triola, G., Waldmann, H., Goody, R. S., & Wu, Y-W. (2011). One-pot dual-labeling of a protein by two chemoselective reactions. Angewandte Chemie - International Edition, 50(36), 8287-8290. https://doi.org/10.1002/anie.201100840

Yi L, Sun H, Itzen A, Triola G, Waldmann H, Goody RS et al. One-pot dual-labeling of a protein by two chemoselective reactions. Angewandte Chemie - International Edition. 2011 Aug 29;50(36):8287-8290. doi: 10.1002/anie.201100840

Yi, Long ; Sun, Hongyan ; Itzen, Aymelt et al. / One-pot dual-labeling of a protein by two chemoselective reactions. In: Angewandte Chemie - International Edition. 2011 ; Vol. 50, No. 36. pp. 8287-8290.