One-pot dual-labeling of a protein by two chemoselective reactions

Long Yi; Hongyan Sun; Aymelt Itzen; Gemma Triola; Herbert Waldmann; Roger S. Goody; Yao-Wen Wu

doi:10.1002/anie.201100840

One-pot dual-labeling of a protein by two chemoselective reactions

Long Yi
, Hongyan Sun
, Aymelt Itzen
, Gemma Triola
, Herbert Waldmann
, Roger S. Goody
, Yao-Wen Wu

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

35 Citations (Scopus)

Abstract

Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	8287-8290
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	36
DOIs	https://doi.org/10.1002/anie.201100840
Publication status	Published - 29 Aug 2011
Externally published	Yes

Research Keywords

chemoselective reactions
fluorescent labeling
native chemical ligation
oxime ligation
site-specific modification

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10.1002/anie.201100840

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abstract = "Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. {\textcopyright} 2011 Wiley-VCH Verlag GmbH \& Co. KGaA, Weinheim.",

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T1 - One-pot dual-labeling of a protein by two chemoselective reactions

AU - Yi, Long

AU - Sun, Hongyan

AU - Itzen, Aymelt

AU - Triola, Gemma

AU - Waldmann, Herbert

AU - Goody, Roger S.

AU - Wu, Yao-Wen

PY - 2011/8/29

Y1 - 2011/8/29

N2 - Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - chemoselective reactions

KW - fluorescent labeling

KW - native chemical ligation

KW - oxime ligation

KW - site-specific modification

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M3 - RGC 21 - Publication in refereed journal

C2 - 21761514

SN - 1433-7851

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EP - 8290

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 36

ER -

One-pot dual-labeling of a protein by two chemoselective reactions

Abstract

Research Keywords

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