TY - JOUR
T1 - One-pot dual-labeling of a protein by two chemoselective reactions
AU - Yi, Long
AU - Sun, Hongyan
AU - Itzen, Aymelt
AU - Triola, Gemma
AU - Waldmann, Herbert
AU - Goody, Roger S.
AU - Wu, Yao-Wen
PY - 2011/8/29
Y1 - 2011/8/29
N2 - Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
AB - Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
KW - chemoselective reactions
KW - fluorescent labeling
KW - native chemical ligation
KW - oxime ligation
KW - site-specific modification
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-80052077967&origin=recordpage
U2 - 10.1002/anie.201100840
DO - 10.1002/anie.201100840
M3 - RGC 21 - Publication in refereed journal
C2 - 21761514
SN - 1433-7851
VL - 50
SP - 8287
EP - 8290
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 36
ER -