One Polyketide Synthase, Two Distinct Products : Trans‐Acting Enzyme‐Controlled Product Divergence in Calbistrin Biosynthesis
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
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Detail(s)
Original language | English |
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Pages (from-to) | 8851-8858 |
Journal / Publication | Angewandte Chemie - International Edition |
Volume | 60 |
Issue number | 16 |
Online published | 22 Jan 2021 |
Publication status | Published - 12 Apr 2021 |
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Abstract
Calbistrins are fungal polyketides consisting of the characteristic decalin and polyene moieties. Although the biosynthetic gene cluster of calbistrin A was recently identified, the pathway of calbistrin A biosynthesis has largely remained uninvestigated. Herein, we investigated the mechanism by which the backbone structures of calbistrins are formed, by heterologous and in vitro reconstitution of the biosynthesis and a structural biological study. Intriguingly, our analyses revealed that the decalin and polyene portions of calbistrins are synthesized by the single polyketide synthase (PKS) CalA, with the aid of the trans‐acting enoylreductase CalK and the trans‐acting methyltransferase CalH, respectively. We also determined that the esterification of the two polyketide parts is catalyzed by the acyltransferase CalD. Our study has uncovered a novel dual‐functional PKS and thus broadened our understanding of how fungi synthesize diverse polyketide natural products.
Research Area(s)
- biosynthesis, calbistrins, natural products, polyketide synthases, polyketides
Citation Format(s)
One Polyketide Synthase, Two Distinct Products : Trans‐Acting Enzyme‐Controlled Product Divergence in Calbistrin Biosynthesis. / Tao, Hui; Mori, Takahiro; Wei, Xingxing et al.
In: Angewandte Chemie - International Edition, Vol. 60, No. 16, 12.04.2021, p. 8851-8858.Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review