Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes

Lawrence Petherbridge; Andrew C. Brown; Susan J. Baigent; Ken Howes; Melanie A. Sacco; Nikolaus Osterrieder; Venugopal K. Nair

doi:10.1128/JVI.78.23.13376-13380.2004

Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes

Lawrence Petherbridge
, Andrew C. Brown
, Susan J. Baigent
, Ken Howes
, Melanie A. Sacco
, Nikolaus Osterrieder
, Venugopal K. Nair

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

113 Citations (Scopus)

Abstract

Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones of the highly oncogenic RB-1B strain by inserting mini-F vector sequences into the U_S2 locus. MDV reconstituted from two BAC clones induced rapid-onset lymphomas similar to those induced by the wild-type virus. Virus reconstituted from another BAC clone that showed a 7.7-kbp deletion in the internal and terminal unique long repeat regions was nononcogenic, suggesting that the deleted region may be associated with oncogenicity. The generation of the oncogenic BAC clones of MDV is a significant step in unraveling the oncogenic determinants of this virus.

Original language	English
Pages (from-to)	13376-13380
Journal	Journal of Virology
Volume	78
Issue number	23
Online published	12 Nov 2004
DOIs	https://doi.org/10.1128/JVI.78.23.13376-13380.2004
Publication status	Published - Dec 2004
Externally published	Yes

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SDG 3 Good Health and Well-being

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@article{67984ec575bb48afa2ad7e78c8b5d0e7,

title = "Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes",

abstract = "Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones of the highly oncogenic RB-1B strain by inserting mini-F vector sequences into the US2 locus. MDV reconstituted from two BAC clones induced rapid-onset lymphomas similar to those induced by the wild-type virus. Virus reconstituted from another BAC clone that showed a 7.7-kbp deletion in the internal and terminal unique long repeat regions was nononcogenic, suggesting that the deleted region may be associated with oncogenicity. The generation of the oncogenic BAC clones of MDV is a significant step in unraveling the oncogenic determinants of this virus.",

author = "Lawrence Petherbridge and Brown, \{Andrew C.\} and Baigent, \{Susan J.\} and Ken Howes and Sacco, \{Melanie A.\} and Nikolaus Osterrieder and Nair, \{Venugopal K.\}",

year = "2004",

month = dec,

doi = "10.1128/JVI.78.23.13376-13380.2004",

language = "English",

volume = "78",

pages = "13376--13380",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "23",

}

TY - JOUR

T1 - Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes

AU - Petherbridge, Lawrence

AU - Brown, Andrew C.

AU - Baigent, Susan J.

AU - Howes, Ken

AU - Sacco, Melanie A.

AU - Osterrieder, Nikolaus

AU - Nair, Venugopal K.

PY - 2004/12

Y1 - 2004/12

N2 - Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones of the highly oncogenic RB-1B strain by inserting mini-F vector sequences into the US2 locus. MDV reconstituted from two BAC clones induced rapid-onset lymphomas similar to those induced by the wild-type virus. Virus reconstituted from another BAC clone that showed a 7.7-kbp deletion in the internal and terminal unique long repeat regions was nononcogenic, suggesting that the deleted region may be associated with oncogenicity. The generation of the oncogenic BAC clones of MDV is a significant step in unraveling the oncogenic determinants of this virus.

AB - Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones of the highly oncogenic RB-1B strain by inserting mini-F vector sequences into the US2 locus. MDV reconstituted from two BAC clones induced rapid-onset lymphomas similar to those induced by the wild-type virus. Virus reconstituted from another BAC clone that showed a 7.7-kbp deletion in the internal and terminal unique long repeat regions was nononcogenic, suggesting that the deleted region may be associated with oncogenicity. The generation of the oncogenic BAC clones of MDV is a significant step in unraveling the oncogenic determinants of this virus.

UR - https://www.scopus.com/pages/publications/8644241688

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-8644241688&origin=recordpage

U2 - 10.1128/JVI.78.23.13376-13380.2004

DO - 10.1128/JVI.78.23.13376-13380.2004

M3 - RGC 21 - Publication in refereed journal

C2 - 15542691

SN - 0022-538X

VL - 78

SP - 13376

EP - 13380

JO - Journal of Virology

JF - Journal of Virology

IS - 23

ER -

Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes

Abstract

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