On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood

Lien-Yu Hung; Wei-Chun Tsai; Chien-Yu Fu; Nai-Jung Chiang; Yu-Chun Wang; Yan-Shen Shan; Gwo-Bin Lee

On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood

Lien-Yu Hung
, Wei-Chun Tsai
, Chien-Yu Fu
, Nai-Jung Chiang
, Yu-Chun Wang
, Yan-Shen Shan
, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	563-564
ISBN (Print)	9780979806490
Publication status	Published - 2016
Externally published	Yes
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Publication series

Name	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Cholangiocarcinoma (CCA)
Immunofluorescence staining
Microfluidics

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

Hung, L.-Y., Tsai, W.-C., Fu, C.-Y., Chiang, N.-J., Wang, Y.-C., Shan, Y.-S., & Lee, G.-B. (2016). On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 563-564). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Hung, Lien-Yu ; Tsai, Wei-Chun ; Fu, Chien-Yu et al. / On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 563-564 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

@inproceedings{de52de15c15e4ca4ab11cabf3ce0a3e7,

title = "On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood",

abstract = "Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.",

keywords = "Cholangiocarcinoma (CCA), Immunofluorescence staining, Microfluidics",

author = "Lien-Yu Hung and Wei-Chun Tsai and Chien-Yu Fu and Nai-Jung Chiang and Yu-Chun Wang and Yan-Shen Shan and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) ; Conference date: 09-10-2016 Through 13-10-2016",

year = "2016",

language = "English",

isbn = "9780979806490",

series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

pages = "563--564",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

}

Hung, L-Y, Tsai, W-C, Fu, C-Y, Chiang, N-J, Wang, Y-C, Shan, Y-S & Lee, G-B 2016, On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 563-564, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), Dublin, Ireland, 9/10/16.

On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. / Hung, Lien-Yu; Tsai, Wei-Chun; Fu, Chien-Yu et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 563-564 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood

AU - Hung, Lien-Yu

AU - Tsai, Wei-Chun

AU - Fu, Chien-Yu

AU - Chiang, Nai-Jung

AU - Wang, Yu-Chun

AU - Shan, Yan-Shen

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2016

Y1 - 2016

N2 - Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.

AB - Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.

KW - Cholangiocarcinoma (CCA)

KW - Immunofluorescence staining

KW - Microfluidics

UR - https://www.scopus.com/pages/publications/85014194666

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85014194666&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9780979806490

T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

SP - 563

EP - 564

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

Y2 - 9 October 2016 through 13 October 2016

ER -

Hung LY, Tsai WC, Fu CY, Chiang NJ, Wang YC, Shan YS et al. On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 563-564. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this