Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

View graph of relations

Author(s)

  • Mingyue Cui
  • Mengjia Zheng
  • Christian Wiraja
  • Sharon Wan Ting Chew
  • Arti Mishra
  • Venkatesh Mayandi
  • Rajamani Lakshminarayanan

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number2102327
Journal / PublicationAdvanced Science
Volume8
Issue number21
Online published8 Sep 2021
Publication statusPublished - 3 Nov 2021

Link(s)

Abstract

The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long-term storage studies, and they successfully impede the growth of gram-negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders.

Research Area(s)

  • cryomicroneedles, drug delivery, eye infection, ocular disease, predatory bacteria

Citation Format(s)

Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection. / Cui, Mingyue; Zheng, Mengjia; Wiraja, Christian; Chew, Sharon Wan Ting; Mishra, Arti; Mayandi, Venkatesh; Lakshminarayanan, Rajamani; Xu, Chenjie.

In: Advanced Science, Vol. 8, No. 21, 2102327, 03.11.2021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

Download Statistics

No data available