Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57

Mengjie Wang; Qi Dong; Hua Wang; Yaqing He; Ying Chen; Hong Zhang; Rong Wu; Xinchun Chen; Boping Zhou; Jason He; Hsiang-Fu Kung; Canhua Huang; Yuquan Wei; Jian-dong Huang; Hongxi Xu; Ming-Liang He

doi:10.18632/oncotarget.7122

Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57

Mengjie Wang
, Qi Dong
, Hua Wang
, Yaqing He
, Ying Chen
, Hong Zhang
, Rong Wu
, Xinchun Chen
, Boping Zhou
, Jason He
, Hsiang-Fu Kung
, Canhua Huang
, Yuquan Wei
, Jian-dong Huang
, Hongxi Xu^*
, Ming-Liang He^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

28 Citations (Scopus)

59 Downloads (CityUHK Scholars)

Abstract

There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.

Original language	English
Pages (from-to)	8797-8808
Journal	Oncotarget
Volume	7
Issue number	8
Online published	1 Feb 2016
DOIs	https://doi.org/10.18632/oncotarget.7122
Publication status	Published - 23 Feb 2016

Funding

The work was partly supported by The National Basic Research Development Program (Grant No. 2013CB911302), Grants from National Science Foundation of China (81471964), Grants from The Science Technology and Innovation Committee of Shenzhen Municipality (JCYJ20130401172046448, JCYJ20120615085419420, JSGG20151030110921727), and the general research grant of Hong Kong (RGC-GRF Nos. 14105214, 464512, 11100215) to Dr. Ming-Liang HE; and by grant of The Significant New Drug Development from National Major Scientific and Technological Special Project (No. 2013ZX09103002-020) to Dr. Hong-xi Xu.

Research Keywords

enterovirus 71
Oblongifolin M
ERp57
antiviral
NASOPHARYNGEAL CARCINOMA
PROTEOMIC ANALYSIS
ANTIVIRAL ACTIVITY
IN-VITRO
PRENYLATED BENZOYLPHLOROGLUCINOLS
HEPATOCELLULAR-CARCINOMA
SIGNALING PATHWAY
MOUTH-DISEASE
INFECTIONS
XANTHONES

Publisher's Copyright Statement

This full text is made available under CC-BY 3.0. https://creativecommons.org/licenses/by/3.0/

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10.18632/oncotarget.7122

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Wang, M., Dong, Q., Wang, H., He, Y., Chen, Y., Zhang, H., Wu, R., Chen, X., Zhou, B., He, J., Kung, H.-F., Huang, C., Wei, Y., Huang, J., Xu, H., & He, M.-L. (2016). Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57. Oncotarget, 7(8), 8797-8808. https://doi.org/10.18632/oncotarget.7122

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title = "Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57",

abstract = "There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.",

keywords = "enterovirus 71, Oblongifolin M, ERp57, antiviral, NASOPHARYNGEAL CARCINOMA, PROTEOMIC ANALYSIS, ANTIVIRAL ACTIVITY, IN-VITRO, PRENYLATED BENZOYLPHLOROGLUCINOLS, HEPATOCELLULAR-CARCINOMA, SIGNALING PATHWAY, MOUTH-DISEASE, INFECTIONS, XANTHONES",

author = "Mengjie Wang and Qi Dong and Hua Wang and Yaqing He and Ying Chen and Hong Zhang and Rong Wu and Xinchun Chen and Boping Zhou and Jason He and Hsiang-Fu Kung and Canhua Huang and Yuquan Wei and Jian-dong Huang and Hongxi Xu and Ming-Liang He",

year = "2016",

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day = "23",

doi = "10.18632/oncotarget.7122",

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Wang, M, Dong, Q, Wang, H, He, Y, Chen, Y, Zhang, H, Wu, R, Chen, X, Zhou, B, He, J, Kung, H-F, Huang, C, Wei, Y, Huang, J, Xu, H & He, M-L 2016, 'Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57', Oncotarget, vol. 7, no. 8, pp. 8797-8808. https://doi.org/10.18632/oncotarget.7122

Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57. / Wang, Mengjie; Dong, Qi; Wang, Hua et al.
In: Oncotarget, Vol. 7, No. 8, 23.02.2016, p. 8797-8808.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57

AU - Wang, Mengjie

AU - Dong, Qi

AU - Wang, Hua

AU - He, Yaqing

AU - Chen, Ying

AU - Zhang, Hong

AU - Wu, Rong

AU - Chen, Xinchun

AU - Zhou, Boping

AU - He, Jason

AU - Kung, Hsiang-Fu

AU - Huang, Canhua

AU - Wei, Yuquan

AU - Huang, Jian-dong

AU - Xu, Hongxi

AU - He, Ming-Liang

PY - 2016/2/23

Y1 - 2016/2/23

N2 - There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.

AB - There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.

KW - enterovirus 71

KW - Oblongifolin M

KW - ERp57

KW - antiviral

KW - NASOPHARYNGEAL CARCINOMA

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KW - ANTIVIRAL ACTIVITY

KW - IN-VITRO

KW - PRENYLATED BENZOYLPHLOROGLUCINOLS

KW - HEPATOCELLULAR-CARCINOMA

KW - SIGNALING PATHWAY

KW - MOUTH-DISEASE

KW - INFECTIONS

KW - XANTHONES

UR - https://www.scopus.com/pages/publications/84961574357

UR - https://www.scopus.com/pages/publications/84961574357#tab=impact

U2 - 10.18632/oncotarget.7122

DO - 10.18632/oncotarget.7122

M3 - RGC 21 - Publication in refereed journal

C2 - 26848777

SN - 1949-2553

VL - 7

SP - 8797

EP - 8808

JO - Oncotarget

JF - Oncotarget

IS - 8

ER -

Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57

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Research Keywords

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GRF: Investigation of the Molecular Mechanisms of Hsp27 Response to Facilitate Human Enterovirus 71 Infection

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Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

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GRF: Investigation of the Molecular Mechanisms of Hsp27 Response to Facilitate Human Enterovirus 71 Infection

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