Nucleic acid microarrays created in the double-spiral format on a circular microfluidic disk

Hong Chen; Lin Wang; Paul C. H. Li

doi:10.1039/b719846a

Nucleic acid microarrays created in the double-spiral format on a circular microfluidic disk

Hong Chen
, Lin Wang
, Paul C. H. Li^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

51 Citations (Scopus)

Abstract

A microfluidic microarray that is created in the double spiral format has produced a greater density of probes than in our previous report. Using this double-spiral format together with centrifugal pumping for liquid delivery, 384 × 384 hybridization assays have been performed on one circular disk at one time, at the intersections between the spiral channels and spiral probe lines. Each sample was introduced into each inlet reservoir leading to 4 spiral channels and was analyzed independently, and so the hybridization results were self-corrected among the 4 spiral channels. In this work, fast microarray hybridizations have been successfully achieved by using both complementary oligonucleotides as well as PCR products prepared from plant fungal pathogen cultures. © The Royal Society of Chemistry.

Original language	English
Pages (from-to)	826-829
Journal	Lab on a Chip
Volume	8
Issue number	5
DOIs	https://doi.org/10.1039/b719846a
Publication status	Published - 2008
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

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title = "Nucleic acid microarrays created in the double-spiral format on a circular microfluidic disk",

abstract = "A microfluidic microarray that is created in the double spiral format has produced a greater density of probes than in our previous report. Using this double-spiral format together with centrifugal pumping for liquid delivery, 384 × 384 hybridization assays have been performed on one circular disk at one time, at the intersections between the spiral channels and spiral probe lines. Each sample was introduced into each inlet reservoir leading to 4 spiral channels and was analyzed independently, and so the hybridization results were self-corrected among the 4 spiral channels. In this work, fast microarray hybridizations have been successfully achieved by using both complementary oligonucleotides as well as PCR products prepared from plant fungal pathogen cultures. {\textcopyright} The Royal Society of Chemistry.",

author = "Hong Chen and Lin Wang and Li, \{Paul C. H.\}",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

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AU - Chen, Hong

AU - Wang, Lin

AU - Li, Paul C. H.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2008

Y1 - 2008

N2 - A microfluidic microarray that is created in the double spiral format has produced a greater density of probes than in our previous report. Using this double-spiral format together with centrifugal pumping for liquid delivery, 384 × 384 hybridization assays have been performed on one circular disk at one time, at the intersections between the spiral channels and spiral probe lines. Each sample was introduced into each inlet reservoir leading to 4 spiral channels and was analyzed independently, and so the hybridization results were self-corrected among the 4 spiral channels. In this work, fast microarray hybridizations have been successfully achieved by using both complementary oligonucleotides as well as PCR products prepared from plant fungal pathogen cultures. © The Royal Society of Chemistry.

AB - A microfluidic microarray that is created in the double spiral format has produced a greater density of probes than in our previous report. Using this double-spiral format together with centrifugal pumping for liquid delivery, 384 × 384 hybridization assays have been performed on one circular disk at one time, at the intersections between the spiral channels and spiral probe lines. Each sample was introduced into each inlet reservoir leading to 4 spiral channels and was analyzed independently, and so the hybridization results were self-corrected among the 4 spiral channels. In this work, fast microarray hybridizations have been successfully achieved by using both complementary oligonucleotides as well as PCR products prepared from plant fungal pathogen cultures. © The Royal Society of Chemistry.

UR - https://www.scopus.com/pages/publications/42549171365

UR - https://www.scopus.com/pages/publications/42549171365#tab=impact

U2 - 10.1039/b719846a

DO - 10.1039/b719846a

M3 - RGC 21 - Publication in refereed journal

C2 - 18432357

SN - 1473-0197

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SP - 826

EP - 829

JO - Lab on a Chip

JF - Lab on a Chip

IS - 5

ER -

Nucleic acid microarrays created in the double-spiral format on a circular microfluidic disk

Abstract

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