Abstract
BACKGROUND: Bacterial wilt induced by Ralstonia solanacearum is regarded as one of the most devastating diseases. However, excessive and repeated use of the same bactericides has resulted in development of bacterial resistance. Targeting bacterial virulence factors, such as type III secretion system (T3SS), without inhibiting bacterial growth is a possible assay to discover new antimicrobial agents.
RESULTS: In this work, identifying new T3SS inhibitors, a series of mandelic acid derivatives with 2-mercapto-1,3,4-thiazole moiety was synthesized. One of them, F-24, inhibited the transcription of hrpY gene significantly. The presence of this compound obviously attenuated hypersensitive response (HR) without inhibiting bacterial growth of R. solanacearum. The transcription levels of those typical T3SS genes were reduced to various degrees. The test of the ability of F-24 in protecting plants demonstrated that F-24 protected tomato plants against bacterial wilt without restricting the multiplication of R. solanacearum. The mechanism of this T3SS inhibition is through the PhcR-PhcA-PrhG-HrpB pathway.
CONCULSION: The screened F-24 could inhibit R. solanacearum T3SS and showed better inhibitory activity than previously reported inhibitors without affecting the growth of the strain, and F-24 is a compound with good potential in the control of R. solanacearum.
© 2023 Society of Chemical Industry.
RESULTS: In this work, identifying new T3SS inhibitors, a series of mandelic acid derivatives with 2-mercapto-1,3,4-thiazole moiety was synthesized. One of them, F-24, inhibited the transcription of hrpY gene significantly. The presence of this compound obviously attenuated hypersensitive response (HR) without inhibiting bacterial growth of R. solanacearum. The transcription levels of those typical T3SS genes were reduced to various degrees. The test of the ability of F-24 in protecting plants demonstrated that F-24 protected tomato plants against bacterial wilt without restricting the multiplication of R. solanacearum. The mechanism of this T3SS inhibition is through the PhcR-PhcA-PrhG-HrpB pathway.
CONCULSION: The screened F-24 could inhibit R. solanacearum T3SS and showed better inhibitory activity than previously reported inhibitors without affecting the growth of the strain, and F-24 is a compound with good potential in the control of R. solanacearum.
© 2023 Society of Chemical Industry.
| Original language | English |
|---|---|
| Pages (from-to) | 4626-4634 |
| Journal | Pest Management Science |
| Volume | 79 |
| Issue number | 11 |
| Online published | 14 Jul 2023 |
| DOIs | |
| Publication status | Published - Nov 2023 |
Funding
The authors acknowledge the financial support from the National Science Fund for Distinguished Young Scholars of Guangdong Province (2021B1515020107), the National Natural Science Foundation of China (32072450), the International Science and Technology Cooperation Program in Guangdong (2022A0505050060), the Key Realm R&D Program of Guangdong Province (2020B0202090001), and the Foundation of Guangdong Provincial Key Laboratory of High Technology for Plant Protection (Zhizhong2023-02).
Research Keywords
- bacterial wilt
- inhibitor
- mandelic acid derivatives
- mechanism
- Ralstonia solanacearum
- type III secretion system (T3SS)
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