Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas : Identification, prevalence, and risk factors

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Ryan M. Troyer
  • Kathryn R. Stutzman-Rodriguez
  • Scott Carver
  • Caitlin C. Lozano
  • Justin S. Lee
  • Michael R. Lappin
  • Seth P.D. Riley
  • Laurel E.K. Serieys
  • Kenneth A. Logan
  • Linda L. Sweanor
  • Walter M. Boyce
  • T. Winston Vickers
  • Roy McBride
  • Kevin R. Crooks
  • Jesse S. Lewis
  • Mark W. Cunningham
  • Joel Rovnak
  • Sandra L. Quackenbush
  • Sue VandeWoude

Detail(s)

Original languageEnglish
Pages (from-to)3914-3924
Journal / PublicationJournal of Virology
Volume88
Issue number8
Online published26 Mar 2014
Publication statusPublished - Apr 2014
Externally publishedYes

Abstract

Gammaherpesviruses (GHVs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. To identify felid GHVs, we screened domestic cat (Felis catus), bobcat (Lynx rufus), and puma (Puma concolor) blood cell DNA samples from California, Colorado, and Florida using a degenerate pan-GHV PCR. Additional pan-GHV and long-distance PCRs were used to sequence a contiguous 3.4-kb region of each putative virus species, including partial glycoprotein B and DNA polymerase genes. We identified three novel GHVs, each present predominantly in one felid species: Felis catus GHV 1 (FcaGHV1) in domestic cats, Lynx rufus GHV 1 (LruGHV1) in bobcats, and Puma concolor GHV 1 (PcoGHV1) in pumas. To estimate infection prevalence, we developed real-time quantitative PCR assays for each virus and screened additional DNA samples from all three species (n=282). FcaGHV1 was detected in 16% of domestic cats across all study sites. LruGHV1 was detected in 47% of bobcats and 13% of pumas across all study sites, suggesting relatively common interspecific transmission. PcoGHV1 was detected in 6% of pumas, all from a specific region of Southern California. The risk of infection for each host varied with geographic location. Age was a positive risk factor for bobcat LruGHV1 infection, and age and being male were risk factors for domestic cat FcaGHV1 infection. Further characterization of these viruses may have significant health implications for domestic cats and may aid studies of free-ranging felid ecology. © 2014, American Society for Microbiology.

Citation Format(s)

Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas : Identification, prevalence, and risk factors. / Troyer, Ryan M.; Beatty, Julia A.; Stutzman-Rodriguez, Kathryn R.; Carver, Scott; Lozano, Caitlin C.; Lee, Justin S.; Lappin, Michael R.; Riley, Seth P.D.; Serieys, Laurel E.K.; Logan, Kenneth A.; Sweanor, Linda L.; Boyce, Walter M.; Winston Vickers, T.; McBride, Roy; Crooks, Kevin R.; Lewis, Jesse S.; Cunningham, Mark W.; Rovnak, Joel; Quackenbush, Sandra L.; VandeWoude, Sue.

In: Journal of Virology, Vol. 88, No. 8, 04.2014, p. 3914-3924.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal