Non-invasive in vivo imaging of the ionic regimes along the gastrointestinal tract of a freshwater vertebrate model organism (Japanese medaka) using responsive photonic crystal beads

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

17 Scopus Citations
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Author(s)

  • Xuemin Du
  • Ngai-Yu Lei
  • Hiu-Man Chiu
  • Xuewu Ge
  • Zhicheng Zhang
  • And 1 others
  • Michael Hon-Wah Lam

Detail(s)

Original languageEnglish
Pages (from-to)1535-1540
Journal / PublicationJournal of Materials Chemistry B
Volume1
Issue number11
Publication statusPublished - 21 Mar 2013

Abstract

Microspherical photonic colloidal crystalline beads that are responsive to media ionic strength of cationic electrolytes have been developed for in vivo imaging of the morphology and concentration gradient of cationic electrolytes along the gastrointestinal (GI) tract of live Japanese medaka (Oryzias latipes). These responsive photonic beads were assembled from core-shell nano-sized particles with polystyrene-co-polyacrylic acid (PS-co-PAA) cores and poly(hydroxyethyl methacrylate-co-p-styrene sulfonate) (PHEMA-co-PSS) hydrogel shells. The three-dimensional orderly packing of these nano-sized core-shell particles gave rise to the photonic properties of the resultant colloidal crystalline array of microspheres. The cationic electrolyte-induced volume phase transition of the sulfonate-laden hydrogel shells of the nano-sized particles altered the lattice spacing among those particles and brought about the photonic responses of the colloidal crystalline beads. Unambiguous changes in the diffraction colour of the colloidal crystalline beads were observable under ordinary ambient light in solution media of increasing concentration of sodium chloride up to 500 mM. These photonic colloidal crystalline beads were found to possess enough structural integrity for in vivo imaging of the GI tract of live Japanese medaka. With the use of a conventional optical microscope, the gradient in the ionic strength of cationic electrolytes along the GI tract of live Japanese medaka larvae was readily revealed, with a lower electrolyte concentration in the mid-intestine (

Citation Format(s)

Non-invasive in vivo imaging of the ionic regimes along the gastrointestinal tract of a freshwater vertebrate model organism (Japanese medaka) using responsive photonic crystal beads. / Du, Xuemin; Lei, Ngai-Yu; Chiu, Hiu-Man et al.
In: Journal of Materials Chemistry B, Vol. 1, No. 11, 21.03.2013, p. 1535-1540.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review