Non-genomic effects of ginsenoside-Re in endothelial cells via glucocorticoid receptor
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 2423-2428 |
Journal / Publication | FEBS Letters |
Volume | 581 |
Issue number | 13 |
Publication status | Published - 29 May 2007 |
Externally published | Yes |
Link(s)
Abstract
We demonstrated that ginsenoside-Re (Re), a pharmacological active component of ginseng, is a functional ligand of glucocorticoid receptor (GR) using competitive ligand-binding assay (IC50 = 156.6 nM; Kd = 49.7 nM) and reporter gene assay. Treatment with Re (1 μM) raises intracellular Ca2+ ([Ca2+]i) and nitric oxide (NO) levels in human umbilical vein endothelial cells as measured using fura-2 and 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate, respectively. Western blot analysis shows that Re increased phosphorylation of endothelial nitric oxide synthase. These effects were abolished by GR antagonist RU486, siRNA targeting GR, non-selective cation channel blocker 2-aminoethyldiphenylborate, or in the absence of extracellular Ca2+, indicating Re is indeed an agonistic ligand for the GR and the activated GR induces rapid Ca2+ influx and NO production in endothelial cells. © 2007.
Research Area(s)
- Angiogenesis, Endothelial cell, Ginsenoside, Glucocorticoid receptor, Intracellular calcium concentration, Nitric oxide
Bibliographic Note
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Citation Format(s)
Non-genomic effects of ginsenoside-Re in endothelial cells via glucocorticoid receptor. / Leung, Kar Wah; Leung, Fung Ping; Huang, Yu et al.
In: FEBS Letters, Vol. 581, No. 13, 29.05.2007, p. 2423-2428.
In: FEBS Letters, Vol. 581, No. 13, 29.05.2007, p. 2423-2428.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review