TY - JOUR
T1 - Non-blinking, highly luminescent, pH- and heavy-metal-ion-stable organic nanodots for bio-imaging
AU - An, Fei-Fei
AU - Ye, Jun
AU - Zhang, Jin-Feng
AU - Yang, Yin-Long
AU - Zheng, Cai-Jun
AU - Zhang, Xiu-Juan
AU - Liu, Zhuang
AU - Lee, Chun-Sing
AU - Zhang, Xiao-Hong
PY - 2013/7/7
Y1 - 2013/7/7
N2 - Heavy-metal-free, organic-small-molecule-based fluorescent nanodots (Sdots, 31-60 nm in diameter) based on 2,7-di(4-(diphenylamino)phenyl-2,1,3- benzothiadiazol-7-yl)-9,9′-spirobifluorene (Spiro-BTA) were prepared through a simple solution process. The Sdots show not only non-blinking and high-brightness fluorescence but also stability in various pH conditions and heavy metal ion solutions. More importantly, Spiro-BTA Sdots demonstrate obviously much higher brightness with very high signal-to-background ratio in Hela cell, compared with common CdSe/ZnS QDs. In addition, they exhibit large Stokes shifts, broad absorption spectra, and low toxicity to living cells which enable their applications as good fluorescence probes for bio-imaging. Further application of Sdots for folate receptor-mediated live-cell endocytosis was demonstrated by non-convalent modification with folic acid linked multidentate ligands. © The Royal Society of Chemistry.
AB - Heavy-metal-free, organic-small-molecule-based fluorescent nanodots (Sdots, 31-60 nm in diameter) based on 2,7-di(4-(diphenylamino)phenyl-2,1,3- benzothiadiazol-7-yl)-9,9′-spirobifluorene (Spiro-BTA) were prepared through a simple solution process. The Sdots show not only non-blinking and high-brightness fluorescence but also stability in various pH conditions and heavy metal ion solutions. More importantly, Spiro-BTA Sdots demonstrate obviously much higher brightness with very high signal-to-background ratio in Hela cell, compared with common CdSe/ZnS QDs. In addition, they exhibit large Stokes shifts, broad absorption spectra, and low toxicity to living cells which enable their applications as good fluorescence probes for bio-imaging. Further application of Sdots for folate receptor-mediated live-cell endocytosis was demonstrated by non-convalent modification with folic acid linked multidentate ligands. © The Royal Society of Chemistry.
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U2 - 10.1039/c3tb20271b
DO - 10.1039/c3tb20271b
M3 - RGC 21 - Publication in refereed journal
SN - 2050-7518
VL - 1
SP - 3144
EP - 3151
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 25
ER -