Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Wei-Cheng Wu
  • Hsin-Yi Tiffany Chen
  • Shih-Chi Lin
  • Hsin-Ying Chen
  • Huan-Tsung Chang
  • Fan-Gang Tseng

Detail(s)

Original languageEnglish
Article number166
Journal / PublicationMicrochimica Acta
Volume186
Issue number3
Online published9 Feb 2019
Publication statusPublished - Mar 2019
Externally publishedYes

Abstract

Stable and low-cost carbon dots (C-dots) were prepared from polyethylenimine (PEI) by a hydrothermal method. It is found that the fluorescence of the C-dots (best measured at excitation/emission wavelengths of 365/473 nm) is quenched by selective oxidation of surface PEI by periodate but recovers in the presence of uric acid (UA). It is assumed that this is due to the selective reduction of the nitrone groups to hydroxylamine groups by UA. The findings were used to design a fluorometric method for determination of UA that has a 2.3 nM detection limit. This is lower than that of reported fluorometric and enzymatic assays. The performance of the method has been validated by determination of UA in samples of human saliva. It is found that the results agree well with those obtained by a commercial UA assay. © 2019, Springer-Verlag GmbH Austria, part of Springer Nature.

Research Area(s)

  • Antioxidant, C-dots, Hydrothermal synthesis, Nitrone, Periodate, Uricase

Citation Format(s)

Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid. / Wu, Wei-Cheng; Chen, Hsin-Yi Tiffany; Lin, Shih-Chi; Chen, Hsin-Ying; Chen, Fu-Rong; Chang, Huan-Tsung; Tseng, Fan-Gang.

In: Microchimica Acta, Vol. 186, No. 3, 166, 03.2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review