TY - JOUR
T1 - Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins
AU - Xu, Chenjie
AU - Xu, Keming
AU - Gu, Hongwei
AU - Zhong, Xiaofen
AU - Guo, Zhihong
AU - Zheng, Rongkun
AU - Zhang, Xixiang
AU - Xu, Bing
PY - 2004/3/1
Y1 - 2004/3/1
N2 - Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins. Copyright © 2004 American Chemical Society.
AB - Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins. Copyright © 2004 American Chemical Society.
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-1642355317&origin=recordpage
U2 - 10.1021/ja031776d
DO - 10.1021/ja031776d
M3 - RGC 21 - Publication in refereed journal
C2 - 15025444
SN - 0002-7863
VL - 126
SP - 3392
EP - 3393
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 11
ER -