TY - JOUR
T1 - Nitric Oxide Nanosensors for Predicting the Development of Osteoarthritis in Rat Model
AU - Jin, Pan
AU - Wiraja, Christian
AU - Zhao, Jinmin
AU - Zhang, Jinlu
AU - Zheng, Li
AU - Xu, Chenjie
PY - 2017/8/2
Y1 - 2017/8/2
N2 - Osteoarthritis (OA) is a chronic arthritic disease that causes the overproduction of inflammatory factors such as nitric oxide (NO). This study develops a NO nanosensor to predict the OA development. The nanosensor is synthesized by encapsulating the NO sensing molecules (i.e., 4-amino-5-methylamino-2′,7′-difluorofluorescein Diaminofluorescein-FM (DAF-FM)) within the biodegradable poly(lactic-co-glycolic acid) nanoparticles. In vitro, the nanosensor allows the monitoring of the NO release in interleukin-1β-stimulated chondrocytes and the alleviated effect of NG-monomethyl-L-arginine (a NO inhibitor) and andrographolide (an anti-inflammatory agent). In the rat OA model, it permits the quantification of NO level in joint fluid. The proposed NO nanosensor may facilitate a noninvasive and real-time evaluation of the OA development.
AB - Osteoarthritis (OA) is a chronic arthritic disease that causes the overproduction of inflammatory factors such as nitric oxide (NO). This study develops a NO nanosensor to predict the OA development. The nanosensor is synthesized by encapsulating the NO sensing molecules (i.e., 4-amino-5-methylamino-2′,7′-difluorofluorescein Diaminofluorescein-FM (DAF-FM)) within the biodegradable poly(lactic-co-glycolic acid) nanoparticles. In vitro, the nanosensor allows the monitoring of the NO release in interleukin-1β-stimulated chondrocytes and the alleviated effect of NG-monomethyl-L-arginine (a NO inhibitor) and andrographolide (an anti-inflammatory agent). In the rat OA model, it permits the quantification of NO level in joint fluid. The proposed NO nanosensor may facilitate a noninvasive and real-time evaluation of the OA development.
KW - DAF-FM
KW - nanosensor
KW - nitric oxide sensing
KW - osteoarthritis
KW - PLGA
UR - http://www.scopus.com/inward/record.url?scp=85026844878&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85026844878&origin=recordpage
U2 - 10.1021/acsami.7b06404
DO - 10.1021/acsami.7b06404
M3 - RGC 21 - Publication in refereed journal
C2 - 28691484
SN - 1944-8244
VL - 9
SP - 25128
EP - 25137
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 30
ER -