NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA

Shan Jiang; Yong Zhang; Kian Meng Lim; Eugene K.W. Sim; Lei Ye

doi:10.1088/0957-4484/20/15/155101

NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA

Shan Jiang
, Yong Zhang
, Kian Meng Lim
, Eugene K.W. Sim
, Lei Ye

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

152 Citations (Scopus)

Abstract

Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF₄ upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules. © 2009 IOP Publishing Ltd.

Original language	English
Article number	155101
Journal	Nanotechnology
Volume	20
Issue number	15
DOIs	https://doi.org/10.1088/0957-4484/20/15/155101
Publication status	Published - 2009
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

The authors acknowledge financial support from A*STAR BMRC (R-397-000-624-305) and the National University of Singapore.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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title = "NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA",

abstract = "Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules. {\textcopyright} 2009 IOP Publishing Ltd.",

author = "Shan Jiang and Yong Zhang and Lim, \{Kian Meng\} and Sim, \{Eugene K.W.\} and Lei Ye",

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TY - JOUR

T1 - NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA

AU - Jiang, Shan

AU - Zhang, Yong

AU - Lim, Kian Meng

AU - Sim, Eugene K.W.

AU - Ye, Lei

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2009

Y1 - 2009

N2 - Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules. © 2009 IOP Publishing Ltd.

AB - Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules. © 2009 IOP Publishing Ltd.

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U2 - 10.1088/0957-4484/20/15/155101

DO - 10.1088/0957-4484/20/15/155101

M3 - RGC 21 - Publication in refereed journal

C2 - 19420539

SN - 0957-4484

VL - 20

JO - Nanotechnology

JF - Nanotechnology

IS - 15

M1 - 155101

ER -

NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA

Abstract

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Funding

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