Next-generation diagnostic test for dengue virus detection using an ultrafast plasmonic colorimetric RT-PCR strategy

Kunlun Jiang; Jung-Hoon Lee; To Sing Fung; Jingrui Wu; Congnuan Liu; Hua Mi; R.P.V. Jayanthe Rajapakse; Udeni B.R. Balasuriya; Yung-Kang Peng; Yun Young Go

doi:10.1016/j.aca.2023.341565

Author(s)

To Sing Fung
Jingrui Wu
R.P.V. Jayanthe Rajapakse
Udeni B.R. Balasuriya
Yun Young Go

Related Research Unit(s)

Detail(s)

Original language	English
Article number	341565
Number of pages	7
Journal / Publication	Analytica Chimica Acta
Volume	1274
Online published	26 Jun 2023
Publication status	Published - 15 Sept 2023

Link(s)

DOI	DOI https://doi.org/10.1016/j.aca.2023.341565 Final Published version
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Attachment(s)	Documents 158546697.pdf Final Published version, 3.47 MB, PDF Publisher's Copyright Statement This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85163831976&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(95298b29-4a24-4400-8591-598b25c61157).html

Abstract

The current global COVID-19 pandemic once again highlighted the urgent need for a simple, cost-effective, and sensitive diagnostic platform that can be rapidly developed for distribution and easy access in resource-limited areas. Here, we present a simple and low-cost plasmonic photothermal (PPT)-reverse transcription-colorimetric polymerase chain reaction (RTcPCR) for molecular diagnosis of dengue virus (DENV) infection. The assay can be completed within 54 min with an estimated detection limit of 1.6 copies/μL of viral nucleic acid. The analytical sensitivity and specificity of PPT-RTcPCR were comparable to that of the reference RT-qPCR assay. Moreover, the clinical performance of PPT-RTcPCR was evaluated and validated using 158 plasma samples collected from patients suspected of dengue infection. The results showed a diagnostic agreement of 97.5% compared to the reference RT-qPCR and demonstrated a clinical sensitivity and specificity of 97.0% and 100%, respectively. The simplicity and reliability of our PPT-RTcPCR strategy suggest it can provide a foundation for developing a field-deployable diagnostic assay for dengue and other infectious diseases. © 2023 The Authors. Published by Elsevier B.V.