Next-generation diagnostic test for dengue virus detection using an ultrafast plasmonic colorimetric RT-PCR strategy

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • To Sing Fung
  • Jingrui Wu
  • R.P.V. Jayanthe Rajapakse
  • Udeni B.R. Balasuriya
  • Yun Young Go

Detail(s)

Original languageEnglish
Article number341565
Number of pages7
Journal / PublicationAnalytica Chimica Acta
Volume1274
Online published26 Jun 2023
Publication statusPublished - 15 Sept 2023

Link(s)

Abstract

The current global COVID-19 pandemic once again highlighted the urgent need for a simple, cost-effective, and sensitive diagnostic platform that can be rapidly developed for distribution and easy access in resource-limited areas. Here, we present a simple and low-cost plasmonic photothermal (PPT)-reverse transcription-colorimetric polymerase chain reaction (RTcPCR) for molecular diagnosis of dengue virus (DENV) infection. The assay can be completed within 54 min with an estimated detection limit of 1.6 copies/μL of viral nucleic acid. The analytical sensitivity and specificity of PPT-RTcPCR were comparable to that of the reference RT-qPCR assay. Moreover, the clinical performance of PPT-RTcPCR was evaluated and validated using 158 plasma samples collected from patients suspected of dengue infection. The results showed a diagnostic agreement of 97.5% compared to the reference RT-qPCR and demonstrated a clinical sensitivity and specificity of 97.0% and 100%, respectively. The simplicity and reliability of our PPT-RTcPCR strategy suggest it can provide a foundation for developing a field-deployable diagnostic assay for dengue and other infectious diseases. © 2023 The Authors. Published by Elsevier B.V.

Research Area(s)

  • Biosensor, Colorimetric, Dengue diagnosis, Photothermal effect, Plasmonic photothermal PCR

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