Network analysis : Acoustic energy transmission detection of polynucleotide hybridization at the sensor-liquid interface

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Hongbo Su
  • Mengsu Yang
  • Krishna M. R. Kallury
  • Michael Thompson

Detail(s)

Original languageEnglish
Pages (from-to)309-312
Journal / PublicationThe Analyst
Volume118
Issue number3
Publication statusPublished - 1993
Externally publishedYes

Abstract

Palladium has been sputtered onto the surface of gold-plated thickness-shear mode acoustic wave sensors. Adsorption of polynucleotides onto the PdO surface is confirmed by X-ray photoelectron spectroscopy and by gas-phase sensor measurements. Hybridization of complementary strands of polynucleotide at the device-liquid interface results in series resonant frequency signals (network analysis) that are significantly higher than expected from mass-based responses. This observation is interpreted in terms of the perturbation of acoustic energy transmission by changes in interfacial properties on hybridization. Preliminary measurements of changes in motional resistance from network analysis are also presented.

Research Area(s)

  • Acoustic wave sensor, Polynucleotide hybridization