Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Penghui Cheng
  • Jianjian Zhang
  • Jiaguo Huang
  • Qingqing Miao
  • Kanyi Pu

Detail(s)

Original languageEnglish
Pages (from-to)6340-6347
Journal / PublicationChemical Science
Volume9
Issue number30
Online published20 Jun 2018
Publication statusPublished - 14 Aug 2018
Externally publishedYes

Link(s)

Abstract

Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of diseases. Although an abnormally high RONS level has been identified in keloids - a benign dermal tumour developed after lesion, the ability of employing RONS probes for keloid detection has not yet been exploited. Herein, we report two near-infrared (NIR) fluorescent probes (CyTF and CyBA) that can specifically distinguish keloid fibroblasts from normal dermal fibroblasts. Both CyTF and CyBA show a 15-fold NIR fluorescence enhancement at 717 nm upon reaction with RONS. However, because CyTF has higher specificity towards ONOO than CyBA, CyTF can detect stimulated fibroblasts in a more sensitive way, showing 3.76 and 2.26-fold fluorescence increments in TGF-β1 stimulated dermal fibroblasts and keloid fibroblasts, respectively. Furthermore, CyTF permits specific detection of implanted keloid fibroblasts in a xenograft live mouse model. Our work thus developed a new optical imaging approach that has the potential for early diagnosis and drug screening of keloids.

Research Area(s)

Citation Format(s)

Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis. / Cheng, Penghui; Zhang, Jianjian; Huang, Jiaguo et al.
In: Chemical Science, Vol. 9, No. 30, 14.08.2018, p. 6340-6347.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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