Near-infrared fluorescence imaging using organic dye nanoparticles

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Jia Yu
  • Xiujuan Zhang
  • Xiaojun Hao
  • Xiaohong Zhang
  • Mengjiao Zhou
  • Xianfeng Chen

Detail(s)

Original languageEnglish
Pages (from-to)3356-3364
Journal / PublicationBiomaterials
Volume35
Issue number10
Publication statusPublished - Mar 2014

Abstract

Near-infrared (NIR) fluorescence imaging in the 700-1000 nm wavelength range has been very attractive for early detection of cancers. Conventional NIR dyes often suffer from limitation of low brightness due to self-quenching, insufficient photo- and bioenvironmental stability, and small Stokes shift. Herein, we present a strategy of using small-molecule organic dye nanoparticles (ONPs) to encapsulate NIR dyes to enable efficient fluorescence resonance energy transfer to obtain NIR probes with remarkably enhanced performance for in vitro and in vivo imaging. In our design, host ONPs are used as not only carriers to trap and stabilize NIR dyes, but also light-harvesting agent to transfer energy to NIR dyes to enhance their brightness. In comparison with pure NIR dyes, our organic dye nanoparticles possess almost 50-fold increased brightness, large Stokes shifts (~250 nm) and dramatically enhanced photostability. With surface modification, these NIR-emissive organic nanoparticles have water-dispersity and size- and fluorescence- stability over pH values from 2 to 10 for almost 60 days. With these superior advantages, these NIR-emissive organic nanoparticles can be used for highly efficient folic-acid aided specific targeting in vivo and ex vivo cellular imaging. Finally, during in vivo imaging, the nanoparticles show negligible toxicity. Overall, the results clearly display a potential application of using the NIR-emissive organic nanoparticles for in vitro and in vivo imaging. © 2014 Elsevier Ltd.

Research Area(s)

  • Fluorescence resonance energy transfer, In vitro imaging, In vivo imaging, NIR-emissive organic nanoparticles

Citation Format(s)

Near-infrared fluorescence imaging using organic dye nanoparticles. / Yu, Jia; Zhang, Xiujuan; Hao, Xiaojun; Zhang, Xiaohong; Zhou, Mengjiao; Lee, Chun-Sing; Chen, Xianfeng.

In: Biomaterials, Vol. 35, No. 10, 03.2014, p. 3356-3364.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review