Near-infrared double-illumination optical-resolution photoacoustic microscopy

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Jiawei Shi
  • Canice Chun-Yin Yiu
  • Can Li
  • Kenneth Kin-Yip Wong

Detail(s)

Original languageEnglish
Article numbere202000392
Journal / PublicationJournal of Biophotonics
Volume14
Issue number3
Online published18 Nov 2020
Publication statusPublished - Mar 2021

Abstract

Label-free chemical bond imaging is of great importance in biology and medicine. Photoacoustic imaging at the third near-infrared windows (1600-1870 nm, near-infrared-III) provides a stable molecular vibrational imaging tool for lipid-rich tissue owing to the first overtone transition of the C-H bond at 1.7 μm. However, lacking high-energy pulsed laser sources at 1.7 μm and the strong water absorption significantly limit the signal-to-noise ratio of the lipid imaging, especially for thin lipid tissues. To circumvent this barrier, we develop near-infrared-III double-illumination optical-resolution photoacoustic microscopy (DIOR-PAM) for improving the sensitivity of label-free lipid imaging. Using the same laser, DIOR-PAM can enhance the sensitivity by nearly 100%, which we prove in the Monte Carlo simulation. We experimentally demonstrated 50% ~ 100% sensitivity enhancements on nonbiological and biological lipid-rich samples.

Research Area(s)

  • near infrared, photoacoustic imaging, sensitivity enhancement, vibration absorption

Citation Format(s)

Near-infrared double-illumination optical-resolution photoacoustic microscopy. / Li, Mingsheng; Shi, Jiawei; Yiu, Canice Chun-Yin; Li, Can; Wong, Kenneth Kin-Yip; Wang, Lidai.

In: Journal of Biophotonics, Vol. 14, No. 3, e202000392, 03.2021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review