NBD-based synthetic probes for sensing small molecules and proteins: design, sensing mechanisms and biological applications

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

5 Scopus Citations
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Author(s)

  • Chenyang Jiang
  • Haojie Huang
  • Xueying Kang
  • Zhen Xi
  • Michael D. Pluth
  • Long Yi

Detail(s)

Original languageEnglish
Pages (from-to)7436–7495
Journal / PublicationChemical Society Reviews
Volume50
Issue number13
Online published1 Jun 2021
Publication statusPublished - 7 Jul 2021

Abstract

Compounds with a nitrobenzoxadiazole (NBD) skeleton exhibit prominent useful properties including environmental sensitivity, high reactivity toward amines and biothiols (including H2S) accompanied by distinct colorimetric and fluorescent changes, fluorescence-quenching ability, and small size, all of which facilitate biomolecular sensing and self-assembly. Amines are important biological nucleophiles, and the unique activity of NBD ethers with amines has allowed for site-specific protein labelling and for the detection of enzyme activities. Both H2S and biothiols are involved in a wide range of physiological processes in mammals, and misregulation of these small molecules is associated with numerous diseases including cancers. In this review, we focus on NBD-based synthetic probes as advanced chemical tools for biomolecular sensing. Specifically, we discuss the sensing mechanisms and selectivity of the probes, the design strategies for multi-reactable multi-quenching probes, and the associated biological applications of these important constructs. We also highlight self-assembled NBD-based probes and outline future directions for NBD-based chemosensors. We hope that this comprehensive review will facilitate the development of future probes for investigating and understanding different biological processes and aid the development of potential theranostic agents.

Research Area(s)

  • INFRARED FLUORESCENT-PROBE, TURN-ON PROBE, CYSTATHIONINE-BETA-SYNTHASE, PUSH-PULL FLUOROPHORE, SMALL D-PEPTIDES, HYDROGEN-SULFIDE, LIVING CELLS, SELECTIVE DETECTION, CANCER-CELLS, OXIDATIVE STRESS

Citation Format(s)

NBD-based synthetic probes for sensing small molecules and proteins: design, sensing mechanisms and biological applications. / Jiang, Chenyang; Huang, Haojie; Kang, Xueying; Yang, Liu; Xi, Zhen; Sun, Hongyan; Pluth, Michael D.; Yi, Long.

In: Chemical Society Reviews, Vol. 50, No. 13, 07.07.2021, p. 7436–7495.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review