Nasopharyngeal Cell Spreading and Migration Characteristics on Microengineered Gratings

Yi Liu; Stella W. Pang; Raymond H. W. Lam

doi:10.1109/ICST46873.2019.9047681

Nasopharyngeal Cell Spreading and Migration Characteristics on Microengineered Gratings

Yi Liu
, Stella W. Pang
, Raymond H. W. Lam^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Physical phenotypic behaviors of cancer cells such as spreading and migration are critical in tumor formation and expanding. On the other hand, these phenotypic behaviors are well proven to be determined by the cell environment. In this work, we adopt microengineered grating structure with ridge widths of 5 μm, 18 μm and a common depth of 15 μm to examine the corresponding migration and spreading of nasopharyngeal epithelial cells and nasopharyngeal carcinoma cells. Areas of cell spreading on the micro-grating substrates are quantified by an imaging technique based on interference reflection microscopy (IRM). The cell migration is characterized by recording cell positions over time, in order to derive migration speed and trajectory of every cell. Further, we investigate correlations between the spreading and migration characteristics. In short, our microengineered grating platform can be further applied in more detailed analyses on cell mechanobiology as well as the mechanotransduction-related mechanistic studies.

Original language	English
Title of host publication	2019 13th International Conference on Sensing Technology (ICST)
Publisher	IEEE
ISBN (Electronic)	978-1-7281-4807-6
ISBN (Print)	978-1-7281-4808-3
DOIs	https://doi.org/10.1109/ICST46873.2019.9047681
Publication status	Published - Dec 2019
Event	13th International Conference on Sensing Technology (ICST 2019) - Macquarie University, Sydney, Australia Duration: 2 Dec 2019 → 4 Dec 2019 https://www.nssn.org.au/upcoming-events/2019/12/2/the-13th-international-conference-on-sensing-technology https://ieeexplore.ieee.org/xpl/conhome/9039856/proceeding

Conference

Conference	13th International Conference on Sensing Technology (ICST 2019)
Place	Australia
City	Sydney
Period	2/12/19 → 4/12/19
Internet address	https://www.nssn.org.au/upcoming-events/2019/12/2/the-13th-international-conference-on-sensing-technology https://ieeexplore.ieee.org/xpl/conhome/9039856/proceeding

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

nasopharyngeal
cancer
spreading
migration
microstructure

Access Output

10.1109/ICST46873.2019.9047681

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@inproceedings{05180ff7410d4d1087525e9d540a3a16,

title = "Nasopharyngeal Cell Spreading and Migration Characteristics on Microengineered Gratings",

abstract = "Physical phenotypic behaviors of cancer cells such as spreading and migration are critical in tumor formation and expanding. On the other hand, these phenotypic behaviors are well proven to be determined by the cell environment. In this work, we adopt microengineered grating structure with ridge widths of 5 μm, 18 μm and a common depth of 15 μm to examine the corresponding migration and spreading of nasopharyngeal epithelial cells and nasopharyngeal carcinoma cells. Areas of cell spreading on the micro-grating substrates are quantified by an imaging technique based on interference reflection microscopy (IRM). The cell migration is characterized by recording cell positions over time, in order to derive migration speed and trajectory of every cell. Further, we investigate correlations between the spreading and migration characteristics. In short, our microengineered grating platform can be further applied in more detailed analyses on cell mechanobiology as well as the mechanotransduction-related mechanistic studies.",

keywords = "nasopharyngeal, cancer, spreading, migration, microstructure",

author = "Yi Liu and Pang, \{Stella W.\} and Lam, \{Raymond H. W.\}",

year = "2019",

month = dec,

doi = "10.1109/ICST46873.2019.9047681",

language = "English",

isbn = "978-1-7281-4808-3",

booktitle = "2019 13th International Conference on Sensing Technology (ICST)",

publisher = "IEEE",

address = "United States",

note = "13th International Conference on Sensing Technology (ICST 2019) ; Conference date: 02-12-2019 Through 04-12-2019",

url = "https://www.nssn.org.au/upcoming-events/2019/12/2/the-13th-international-conference-on-sensing-technology, https://ieeexplore.ieee.org/xpl/conhome/9039856/proceeding",

}

TY - GEN

T1 - Nasopharyngeal Cell Spreading and Migration Characteristics on Microengineered Gratings

AU - Liu, Yi

AU - Pang, Stella W.

AU - Lam, Raymond H. W.

PY - 2019/12

Y1 - 2019/12

N2 - Physical phenotypic behaviors of cancer cells such as spreading and migration are critical in tumor formation and expanding. On the other hand, these phenotypic behaviors are well proven to be determined by the cell environment. In this work, we adopt microengineered grating structure with ridge widths of 5 μm, 18 μm and a common depth of 15 μm to examine the corresponding migration and spreading of nasopharyngeal epithelial cells and nasopharyngeal carcinoma cells. Areas of cell spreading on the micro-grating substrates are quantified by an imaging technique based on interference reflection microscopy (IRM). The cell migration is characterized by recording cell positions over time, in order to derive migration speed and trajectory of every cell. Further, we investigate correlations between the spreading and migration characteristics. In short, our microengineered grating platform can be further applied in more detailed analyses on cell mechanobiology as well as the mechanotransduction-related mechanistic studies.

AB - Physical phenotypic behaviors of cancer cells such as spreading and migration are critical in tumor formation and expanding. On the other hand, these phenotypic behaviors are well proven to be determined by the cell environment. In this work, we adopt microengineered grating structure with ridge widths of 5 μm, 18 μm and a common depth of 15 μm to examine the corresponding migration and spreading of nasopharyngeal epithelial cells and nasopharyngeal carcinoma cells. Areas of cell spreading on the micro-grating substrates are quantified by an imaging technique based on interference reflection microscopy (IRM). The cell migration is characterized by recording cell positions over time, in order to derive migration speed and trajectory of every cell. Further, we investigate correlations between the spreading and migration characteristics. In short, our microengineered grating platform can be further applied in more detailed analyses on cell mechanobiology as well as the mechanotransduction-related mechanistic studies.

KW - nasopharyngeal

KW - cancer

KW - spreading

KW - migration

KW - microstructure

UR - https://www.scopus.com/pages/publications/85083032656

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85083032656&origin=recordpage

U2 - 10.1109/ICST46873.2019.9047681

DO - 10.1109/ICST46873.2019.9047681

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 978-1-7281-4808-3

BT - 2019 13th International Conference on Sensing Technology (ICST)

PB - IEEE

T2 - 13th International Conference on Sensing Technology (ICST 2019)

Y2 - 2 December 2019 through 4 December 2019

ER -

Nasopharyngeal Cell Spreading and Migration Characteristics on Microengineered Gratings

Abstract

Conference

UN SDGs

Research Keywords

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this