Nanozymes Integrated Biochips Toward Smart Detection System

Nanozyme integrated-biochip systems merge the robust catalytic properties of nanozymes with the portability capabilities of biochips, which have demonstrated significant potential for molecular identification and diagnostic applications. Benefiting from the progressive incorporation of artificial intelligence (AI), nanozyme-biochip systems further achieve substantial improvements in both efficiency and accuracy. In this review, recent progress in nanozyme-biochip systems for intelligent detection are summarized. Advancing from fundamental concepts to integrated systems, this overview examines nanozyme-driven signal amplification, biochip-mediated signal presentation, and AI-accelerated signal processing in nanozyme-biochip platforms. Furthermore, the translational potential of nanozyme-biochip systems is illustrated through a critical evaluation of their representative applications in clinical diagnostics, food safety, and environmental monitoring. The current major challenges and future directions in nanozyme-biochip systems are also analyzed, with particular emphasis on AI-assisted development. By integrating advances in nano-catalysis, microdevice engineering, and intelligent computation, this review aims to provide an interdisciplinary roadmap for next-generation biosensing systems. © 2025 The Author(s). Advanced Science published by Wiley-VCH GmbH.