Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics

David J. Peeler; Rujie Sun; Ceren Kütahya; Patrick Peschke; Kun Zhou; Giulia Brachi; Jonathan Yeow; Omar Rifaie-Graham; Jonathan P. Wojciechowski; Thomas F. F. Fernandez Debets; Vernon LaLone; Xin Song; Krunal Polra; Paul F. McKay; John S. Tregoning; Robin J. Shattock; Molly M. Stevens

doi:10.1002/adma.202417290

Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics

David J. Peeler (Co-first Author), Rujie Sun (Co-first Author), Ceren Kütahya, Patrick Peschke, Kun Zhou, Giulia Brachi, Jonathan Yeow, Omar Rifaie-Graham, Jonathan P. Wojciechowski, Thomas F. F. Fernandez Debets, Vernon LaLone, Xin Song, Krunal Polra, Paul F. McKay, John S. Tregoning, Robin J. Shattock, Molly M. Stevens^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

2 Citations (Scopus)

6 Downloads (CityUHK Scholars)

Abstract

Two-photon polymerization (2PP) 3D printing enables top–down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks of Biocompatible polyEsters (SCRIBE) is a novel poly(lactic-co-glycolic acid)-triacrylate resin family with sub-micron resolution and tuneable hydrolysis that addresses the limitations of current 2PP resins. SCRIBE enables the direct printing of hollow microparticles with tuneable chemistry and complex geometries inaccessible to molding techniques, which are used to engineer controlled protein release in vitro and in vivo. SCRIBE microparticles are used to modulate antibody titers and class switching as a function of antigen release rate and extend these findings to enable a single-injection vaccine formulation with extended antibody induction kinetics. Demonstrating how the chemistry and computer-aided design of 2PP-printed microparticles can be used to tune responses to biomacromolecule release in vivo opens significant opportunities for a new generation of drug delivery vehicles. © 2025 The Author(s). Advanced Materials published by Wiley-VCH GmbH.

Original language	English
Article number	2417290
Journal	Advanced Materials
Volume	37
Issue number	15
Online published	28 Feb 2025
DOIs	https://doi.org/10.1002/adma.202417290
Publication status	Published - 16 Apr 2025
Externally published	Yes

Research Keywords

3D printing
immunoengineering
two photon polymerization
vaccine delivery

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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10.1002/adma.202417290

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Peeler, D. J., Sun, R., Kütahya, C., Peschke, P., Zhou, K., Brachi, G., Yeow, J., Rifaie-Graham, O., Wojciechowski, J. P., Fernandez Debets, T. F. F., LaLone, V., Song, X., Polra, K., McKay, P. F., Tregoning, J. S., Shattock, R. J., & Stevens, M. M. (2025). Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics. Advanced Materials, 37(15), Article 2417290. https://doi.org/10.1002/adma.202417290

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title = "Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics",

abstract = "Two-photon polymerization (2PP) 3D printing enables top–down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks of Biocompatible polyEsters (SCRIBE) is a novel poly(lactic-co-glycolic acid)-triacrylate resin family with sub-micron resolution and tuneable hydrolysis that addresses the limitations of current 2PP resins. SCRIBE enables the direct printing of hollow microparticles with tuneable chemistry and complex geometries inaccessible to molding techniques, which are used to engineer controlled protein release in vitro and in vivo. SCRIBE microparticles are used to modulate antibody titers and class switching as a function of antigen release rate and extend these findings to enable a single-injection vaccine formulation with extended antibody induction kinetics. Demonstrating how the chemistry and computer-aided design of 2PP-printed microparticles can be used to tune responses to biomacromolecule release in vivo opens significant opportunities for a new generation of drug delivery vehicles. {\textcopyright} 2025 The Author(s). Advanced Materials published by Wiley-VCH GmbH.",

keywords = "3D printing, immunoengineering, two photon polymerization, vaccine delivery",

author = "Peeler, {David J.} and Rujie Sun and Ceren K{\"u}tahya and Patrick Peschke and Kun Zhou and Giulia Brachi and Jonathan Yeow and Omar Rifaie-Graham and Wojciechowski, {Jonathan P.} and {Fernandez Debets}, {Thomas F. F.} and Vernon LaLone and Xin Song and Krunal Polra and McKay, {Paul F.} and Tregoning, {John S.} and Shattock, {Robin J.} and Stevens, {Molly M.}",

year = "2025",

month = apr,

day = "16",

doi = "10.1002/adma.202417290",

language = "English",

volume = "37",

journal = "Advanced Materials",

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Peeler, DJ, Sun, R, Kütahya, C, Peschke, P, Zhou, K, Brachi, G, Yeow, J, Rifaie-Graham, O, Wojciechowski, JP, Fernandez Debets, TFF, LaLone, V, Song, X, Polra, K, McKay, PF, Tregoning, JS, Shattock, RJ & Stevens, MM 2025, 'Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics', Advanced Materials, vol. 37, no. 15, 2417290. https://doi.org/10.1002/adma.202417290

TY - JOUR

T1 - Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics

AU - Peeler, David J.

AU - Sun, Rujie

AU - Kütahya, Ceren

AU - Peschke, Patrick

AU - Zhou, Kun

AU - Brachi, Giulia

AU - Yeow, Jonathan

AU - Rifaie-Graham, Omar

AU - Wojciechowski, Jonathan P.

AU - Fernandez Debets, Thomas F. F.

AU - LaLone, Vernon

AU - Song, Xin

AU - Polra, Krunal

AU - McKay, Paul F.

AU - Tregoning, John S.

AU - Shattock, Robin J.

AU - Stevens, Molly M.

PY - 2025/4/16

Y1 - 2025/4/16

N2 - Two-photon polymerization (2PP) 3D printing enables top–down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks of Biocompatible polyEsters (SCRIBE) is a novel poly(lactic-co-glycolic acid)-triacrylate resin family with sub-micron resolution and tuneable hydrolysis that addresses the limitations of current 2PP resins. SCRIBE enables the direct printing of hollow microparticles with tuneable chemistry and complex geometries inaccessible to molding techniques, which are used to engineer controlled protein release in vitro and in vivo. SCRIBE microparticles are used to modulate antibody titers and class switching as a function of antigen release rate and extend these findings to enable a single-injection vaccine formulation with extended antibody induction kinetics. Demonstrating how the chemistry and computer-aided design of 2PP-printed microparticles can be used to tune responses to biomacromolecule release in vivo opens significant opportunities for a new generation of drug delivery vehicles. © 2025 The Author(s). Advanced Materials published by Wiley-VCH GmbH.

AB - Two-photon polymerization (2PP) 3D printing enables top–down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks of Biocompatible polyEsters (SCRIBE) is a novel poly(lactic-co-glycolic acid)-triacrylate resin family with sub-micron resolution and tuneable hydrolysis that addresses the limitations of current 2PP resins. SCRIBE enables the direct printing of hollow microparticles with tuneable chemistry and complex geometries inaccessible to molding techniques, which are used to engineer controlled protein release in vitro and in vivo. SCRIBE microparticles are used to modulate antibody titers and class switching as a function of antigen release rate and extend these findings to enable a single-injection vaccine formulation with extended antibody induction kinetics. Demonstrating how the chemistry and computer-aided design of 2PP-printed microparticles can be used to tune responses to biomacromolecule release in vivo opens significant opportunities for a new generation of drug delivery vehicles. © 2025 The Author(s). Advanced Materials published by Wiley-VCH GmbH.

KW - 3D printing

KW - immunoengineering

KW - two photon polymerization

KW - vaccine delivery

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-105002636160&origin=recordpage

U2 - 10.1002/adma.202417290

DO - 10.1002/adma.202417290

M3 - RGC 21 - Publication in refereed journal

C2 - 40018867

SN - 0935-9648

VL - 37

JO - Advanced Materials

JF - Advanced Materials

IS - 15

M1 - 2417290

ER -

Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics

Abstract

Research Keywords

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