Nanoporous membrane based impedance sensors to detect the enzymatic activity of botulinum neurotoxin A

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

15 Scopus Citations
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Detail(s)

Original languageEnglish
Pages (from-to)6544-6550
Journal / PublicationJournal of Materials Chemistry B
Volume1
Issue number47
Online published17 Oct 2013
Publication statusPublished - 21 Dec 2013
Externally publishedYes

Abstract

Botulinum neurotoxins are among the most potent toxic bacterial proteins for humans and there is a great need to develop simple, rapid and sensitive methods for toxin detection and protease activity quantification in field deployment. In this paper, a nanoporous membrane based impedance sensor was developed to monitor the activity of the BoNT serotype A light chain protease (LcA). Synaptosomal-associated protein 25 (SNAP-25) was first immobilized inside nanopore walls via silane linkers. BoNT LcA was then injected over the nanoporous membrane substrate sensor and specifically cleaved SNAP-25. The cleavage activity could be monitored by measuring impedance signals across nanoporous membranes which represented the nanopore blockage degree. This initial device could achieve a 500 pM LcA detection limit within 25 minutes.