Nanopattern-induced changes in morphology and motility of smooth muscle cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Evelyn K.F. Yim
  • Ron M. Reano
  • Albert F. Yee
  • Christopher S. Chen
  • Kam W. Leong

Detail(s)

Original languageEnglish
Pages (from-to)5405-5413
Journal / PublicationBiomaterials
Volume26
Issue number26
Publication statusPublished - Sep 2005
Externally publishedYes

Abstract

Cells are known to be surrounded by nanoscale topography in their natural extracellular environment. The cell behavior, including morphology, proliferation, and motility of bovine pulmonary artery smooth muscle cells (SMC) were studied on poly(methyl methacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) surfaces comprising nanopatterned gratings with 350 nm linewidth, 700 nm pitch, and 350 nm depth. More than 90% of the cells aligned to the gratings, and were significantly elongated compared to the SMC cultured on non-patterned surfaces. The nuclei were also elongated and aligned. Proliferation of the cells was significantly reduced on the nanopatterned surfaces. The polarization of microtubule organizing centers (MTOC), which are associated with cell migration, of SMC cultured on nanopatterned surfaces showed a preference towards the axis of cell alignment in an in vitro wound healing assay. In contrast, the MTOC of SMC on non-patterned surfaces preferentially polarized towards the wound edge. It is proposed that this nanoimprinting technology will provide a valuable platform for studies in cell-substrate interactions and for development of medical devices with nanoscale features. © 2005 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Bionanotechnology, Cell-substrate interaction, Nanoimprinting, Nanomedicine, Nanotopography, Smooth muscle cells

Citation Format(s)

Nanopattern-induced changes in morphology and motility of smooth muscle cells. / Yim, Evelyn K.F.; Reano, Ron M.; Pang, Stella W.; Yee, Albert F.; Chen, Christopher S.; Leong, Kam W.

In: Biomaterials, Vol. 26, No. 26, 09.2005, p. 5405-5413.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal