Nanoliter Fluid Dispensing Based on Microscopic Vision and Laser Range Sensor

In this paper, a comprehensive fluid dispensing approach is proposed for nanoliter fluid dispensing, which is based on multimicroscopic vision and laser range sensor. In the dispensing task, fluid needs to be dispensed into target holes with diameter of 300 μm. Totally, 21 nanoliter fluid is needed for one dispensing task. To precisely control the fluid volume dispensed, an accurate time-pressure dispensing model is developed for a specific dispensing prototype and the fluid dot altitude is measured by laser range sensor to reflect the dispensed fluid volume. An automatic microfluid dispensing system is developed to verify the effectiveness of the proposed fluid dispensing method. Experiments and results demonstrate that: with the proposed dispensing method and system, dispensing accuracy of 100 pl is achieved, and the fluid dot altitude can be controlled with error range less than 2 μm.